Rapid and Visual Detection of Meloidogyne hapla Using Recombinase Polymerase Amplification Combined with a Lateral Flow Dipstick Assay

文献类型： 外文期刊

第一作者： Song, Zhiqiang

作者： Song, Zhiqiang;Yang, Xiai;Zhang, Xiaowei;Luan, Mingbao;Guo, Bing;Liu, Chunjie;Pan, Jiangpeng;Mei, Shiyong

作者机构：

关键词： nematodes; pathogen detection; vegetables

期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）

ISSN： 0191-2917

年卷期： 2021 年 105 卷 9 期

页码：

收录情况： SCI

摘要： The northern root-knot nematode, Meloidogyne hapla, is a biotrophic parasite that infects many crops and causes severe economic losses worldwide. Rapid and accurate detection of M. hapla is crucial for disease forecasting and control. We developed a recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay for rapid detection of M. hapla. The primers and probe were designed based on the effector gene 16D10 sequence and were highly specific to M. hapla. The RPA reaction was performed at a wide range of temperatures from 25 to 45 degrees C within 5 to 25 min, and the amplicon was visualized directly on the LFD within 5 min. The detection limits of the RPA-LFD assay were 10(-3) females and 10(-2) second-stage juveniles/0.5 g of soil, which was 10 times more sensitive than the conventional PCR assay. In addition, the RPA-LFD assay can detect M. hapla from infested plant roots and soil samples, and the entire detection process can be completed within 1.5 h. These results indicate that the RPA-LFD assay is a simple, rapid, specific, sensitive, and visual method that can be used for rapid detection of M. hapla in the field and in resource-limited conditions.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序