Fast identification of the BmNPV infected silkworms by portable NIR spectroscopy and chemometrics

文献类型： 外文期刊

第一作者： Liu, Yihan

作者： Liu, Yihan;Deng, Shuanglin;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui;Li, Yurong;Zhang, Yeshun;Zhang, Guozheng;Yan, Hui

作者机构：

关键词： Portable; NIR spectroscopy; Silkworms; Virus; BmNPV; Identification

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.4； 五年影响因子：3.9 ）

ISSN： 1386-1425

年卷期： 2024 年 314 卷

页码：

收录情况： SCI

摘要： A convenient, low-cost, and rapid detection of BmNPV-infected silkworms is of great significance for the safety of the sericulture industry. In this study, a portable NIR system was used to collect the spectra of normal silkworms and the infected silkworms induced by the administration of Bombyx mori nuclear polyhedrosis virus (BmNPV). Different spectral pretreatment methods were applied, then principal component analysis (PCA), linear discriminant analysis (LDA), and partial least squares discriminant analysis (PLSDA) were used for the classification analysis. The results showed that PCA and LDA were unable to achieve the purpose. For the PLSDA calibration, after the pretreatment of SNV combining 2nd derivative, it had a high identification performance, and obtained low classification errors of 0.023, 0.033, and 0.030 for the calibration set, cross-validation set, and test set, respectively, with higher sensitivity and specificity. Therefore, the BmNPV-infected silkworms can be identified by portable NIR spectroscopy, which will effectively reduce losses for the sericulture industry.

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