CRISPR/Cas9-mediated mutation of nicotinic acetylcholine receptor (nAChR) α subunit confers moderate levels of resistance to spinosad and imidacloprid in the natural predator Coccinella septempunctata without fitness cost

文献类型： 外文期刊

第一作者： Guo, Xingkai

作者： Guo, Xingkai;Zhang, Lisheng;Wang, Mengqing;Li, Yuyan;Shen, Zhongjian;Mao, Jianjun;Zhang, Lisheng;Nolan, Tony

作者机构：

关键词： seven-spot ladybird; Compatibility; Insecticide; integrated pest management (IPM); neonicotinoid

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：5.6； 五年影响因子：5.0 ）

ISSN： 0171-8177

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Chemical insecticides and natural enemies are important components of integrated pest management (IPM) and are usually incompatible in the field environment. The ladybird beetle Coccinella septempunctata is a generalist predator of aphids worldwide. However, its field application is seriously restricted because it is highly susceptible to insecticides. Here, we constructed CRISPR/Cas9-edited C. septempunctata harboring mutations in the nicotinic acetylcholine receptor alpha subunit (nAChR alpha). The C. septempunctata nAChR alpha (Csn alpha) knockout strain (Csn alpha-D7) showed moderate resistance to spinosad (28.56-fold) and imidacloprid (17.28-fold), but no resistance to abamectin. The survival rates of the caged Csn alpha-D7 C. septempunctata treated with spinosad and imidacloprid at field concentrations were significantly higher than the survival rates of the caged wild-type ladybird beetles treated with field label doses of the same insecticides. The Csn alpha-D7 strain exhibited normal growth, development, reproduction, and predation performance compared to wild-type ladybird beetles, suggesting a low fitness cost caused by the Csn alpha mutation. Heritance analysis demonstrated that the resistance to spinosyns in Csn alpha-D7 was autosomal, incompletely recessive, and closely related to the Csn alpha mutation. This study significantly enhanced the compatibility of insecticides with natural enemies using CRISPR/Cas9 technology, contributing to the reduction in insecticide usage and improvement of the ecological environment.

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