Effects of luteinizing hormone receptor activation in immortalized neoplastic canine T lymphocytes

文献类型： 外文期刊

第一作者： Maxwell, Elizabeth

作者： Maxwell, Elizabeth;Dietz, Alexa;Li, Wanli;Li, Wanli;Li, Wanli;Kutzler, Michelle

作者机构：

关键词： cancer; dog; human chorionic gonadotropin; LH receptor; T -cell lymphoma

期刊名称：AMERICAN JOURNAL OF VETERINARY RESEARCH （ 影响因子：1.4； 五年影响因子：1.2 ）

ISSN： 0002-9645

年卷期： 2025 年 86 卷 1 期

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收录情况： SCI

摘要： OBJECTIVE The expression of luteinizing hormone (LH) receptors has been reported in canine nonneoplastic and neoplastic lymphocytes. This study aimed to determine the effect of LH receptor activation in neoplastic canine T lymphocytes. METHODS Cell lines (CLC, CLK, EMA) derived from 3 dogs with primary T-cell lymphoma were used. Cell lines were previously phenotyped and evaluated for LH receptor expression with flow cytometry. Cell lines were stimulated with increas- ing concentrations (0, 4, 400, and 4,000 U/mL) of human chorionic gonadotropin (hCG; an LH receptor agonist), RNA was extracted, cDNA was synthesized, and gene expression was determined using quantitative PCR and the 2-(Delta Delta Ct) method. Cell lines were stimulated with the same increasing concentrations of hCG, and cell proliferation, adhesion, and transmigration were determined using commercial assays. The effects of LH receptor activation were compared between hCG concentrations using a one-way ANOVA. Significance was defined as P < .05. RESULTS LH receptorstimulation increased LH receptor gene expression in the CLC cell line, and there was a trend for increased expression in the EMA cell line but no effect in the CLK cell line. Activation of LH receptors increased proliferation in all 3 cell lines, endothelium adhesion in 2 cell lines (CLC, CLK), and transmigration in 2 cell lines (CLK, EMA). CONCLUSIONS LH receptor stimulation using hCG increased LH receptor gene expression in neoplastic canine T lymphocytes and increased cell proliferation, adhesion, and transmigration. CLINICAL RELEVANCE These findings may provide a physiologic mechanism for the increased incidence of lymphoma reported in dogs with sustained supraphysiologic LH concentrations.

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