Fatty acid binding protein is required for chitin biosynthesis in the wing of Drosophila melanogaster

文献类型： 外文期刊

第一作者： Chen, Jiqiang

作者： Chen, Jiqiang;Zou, Xu;Yang, Qing;Zhu, Weixing;Yang, Qing;Duan, Yanwei;Yang, Qing;Chen, Jiqiang;Zhao, Zhangwu;Merzendorfer, Hans

作者机构：

关键词： Chitin; Chitin biosynthesis; Chitin synthase; Fatty acid binding protein; Insect cuticle

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.421； 五年影响因子：4.774 ）

ISSN： 0965-1748

年卷期： 2022 年 149 卷

页码：

收录情况： SCI

摘要： Chitin, the major structural polysaccharide in arthropods such as insects and mites, is a linear polymer of Nacetylglucosamine units. The growth and development of insects are intimately coupled with chitin biosynthesis. The membrane-bound beta-glycosyltransferase chitin synthase is known to catalyze the key polymerization step of N-acetylglucosamine. However, the additional proteins that might assist chitin synthase during chitin biosynthesis are not well understood. Recently, fatty acid binding protein (Fabp) has been suggested as a candidate that interacts with the chitin synthase Krotzkopf verkehrt (Kkv) in Drosophila melanogaster. Here, using split-ubiquitin membrane yeast two-hybrid and pull-down assays, we have demonstrated that the Fabp-B splice variant physically interacts with Kkv in vitro. The global knockdown of Fabp in D. melanogaster using RNA interference (RNAi) induced lethality at the larval stage. Moreover, in tissue-specific RNAi experiments, silenced Fabp expression in the epidermis and tracheal system caused a lethal larval phenotype. Fabp knockdown in the wings resulted in an abnormal wing development and uneven cuticular surface. In addition to reducing the chitin content in the first longitudinal vein of wings, Fabp silencing also caused the loss of procuticle laminate structures. This study revealed that Fabp plays an important role in chitin synthesis and contributes to a comprehensive understanding of the complex insect chitin biosynthesis.

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