Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0

文献类型： 外文期刊

第一作者： Zhao, Ying

作者： Zhao, Ying;Chen, Chuanhe;Pan, Jianfei;Yang, Shulin;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Pan, Jianfei;Tao, Cong;Wang, Yanfang;Zhao, Ying;Chen, Chuanhe;Yang, Ning;Lam, Sin Man;Shui, Guanghou;Zhao, Jianguo;Zhao, Jianguo

作者机构：

关键词： RNF20; Adipose-muscle crosstalk; Fiber-type switching; LysoPC (16; 0)

期刊名称：CELLULAR AND MOLECULAR LIFE SCIENCES （ 影响因子：8.0； 五年影响因子：8.7 ）

ISSN： 1420-682X

年卷期： 2023 年 80 卷 9 期

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收录情况： SCI

摘要： Both adipose tissue and skeletal muscle are highly dynamic tissues and interact at the metabolic and hormonal levels in response to internal and external stress, and they coordinate in maintaining whole-body metabolic homeostasis. In our previous study, we revealed that adipocyte-specific Rnf20 knockout mice (ASKO mice) exhibited lower fat mass but higher lean mass, providing a good model for investigating the adipose-muscle crosstalk and exploring the effect of the adipocyte Rnf20 gene on the physiology and metabolism of skeletal muscle. Here, we confirmed that ASKO mice exhibited the significantly increased body weight and gastrocnemius muscle weight. Fiber-type switching in the soleus muscle of ASKO mice was observed, as evidenced by the increased number of fast-twitch fibers and decreased number of slow-twitch fibers. Serum metabolites with significant alteration in abundance were identified by metabolomic analysis and the elevated lysophosphatidylcholine 16:0 [LysoPC (16:0)] was observed in ASKO mice. In addition, lipidome analysis of gonadal white adipose tissue revealed a significant increase in LysoPCs and LysoPC (16:0) in ASKO mice. Furthermore, knockdown of Rnf20 gene in 3T3-L1 cells significantly increased the secretion of LysoPC, suggesting that LysoPC might be a critical metabolite in the adipose-muscle crosstalk of ASKO mice. Furthermore, in vitro study demonstrated that LysoPC (16:0) could induce the expression of fast-twitch muscle fibers related genes in differentiated C2C12 cells, indicating its potential role in adipose-muscle crosstalk. Taken together, these findings not only expand our understanding of the biological functions of Rnf20 gene in systemic lipid metabolism, but also provide insight into adipose tissue dysfunction-induced physiological alterations in skeletal muscle.

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