KIF5B-mediated internalization of FMDV promotes virus infection

文献类型： 外文期刊

第一作者： Zhang, Wei

作者： Zhang, Wei;Yang, Fan;Yang, Yang;Cao, Weijun;Shao, Wenhua;Wang, Jiali;Huang, Mengyao;Chen, Zhitong;Zhao, Xiaoyi;Li, Weiwei;Zhu, Zixiang;Zheng, Haixue;Zhang, Wei;Yang, Fan;Yang, Yang;Cao, Weijun;Shao, Wenhua;Wang, Jiali;Huang, Mengyao;Chen, Zhitong;Zhao, Xiaoyi;Li, Weiwei;Zhu, Zixiang;Zheng, Haixue

作者机构：

关键词： FMDV; VP1 protein; KIF5B; Endosome; Clathrin

期刊名称：VIROLOGICA SINICA （ 影响因子：4.3； 五年影响因子：4.3 ）

ISSN： 1674-0769

年卷期： 2024 年 39 卷 3 期

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) is a highly contagious and economically important disease, which is caused by the FMD virus (FMDV). Although the cell receptor for FMDV has been identified, the specific mechanism of FMDV internalization after infection remains unknown. In this study, we found that kinesin family member 5B (KIF5B) plays a vital role during FMDV internalization. Moreover, we confirmed the interaction between KIF5B and FMDV structural protein VP1 by co-immunoprecipitation (Co-IP) and co-localization in FMDV-infected cells. In particular, the stalk [amino acids (aa) 413-678] domain of KIF5B was indispensable for KIF5B-VP1 interaction. Moreover, overexpression of KIF5B dramatically enhanced FMDV replication; consistently, knockdown or knockout of KIF5B suppressed FMDV replication. Furthermore, we also demonstrated that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating. KIF5B also promotes the transmission of viral particles to early and late endosomes during the early stages of infection. In conclusion, our results demonstrate that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating and intracellular transport. This study may provide a new therapeutic target for developing FMDV antiviral drugs.

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