Beta-carotene regulates the biological activity of EGF in IEC6 cells by alleviating the inflammatory process

文献类型： 外文期刊

第一作者： Wang Guoxia

作者： Wang Guoxia;Zhang, Shuai;Lan Hainan;Xin Zheng;Yang Yu

作者机构：

关键词： Inflammation; IEC6 cell; EGF; EGFR

期刊名称：CELL CYCLE （ 影响因子：5.173； 五年影响因子：4.948 ）

ISSN： 1538-4101

年卷期： 2022 年 21 卷 16 期

页码：

收录情况： SCI

摘要： Epidermal growth factor (EGF) has many important biological functions. It plays an important role in regulating the growth, survival, migration, apoptosis, proliferation, and differentiation of intestinal tissues and cells. However, until now, the effect of inflammation on the biological activity of EGF in intestinal cells or tissues is still unclear. For this reason, in the current research, we have conducted a detailed study on this issue. Using the rat small intestinal crypt epithelial cell line (IEC6) was used as an in vitro model, and Confocal laser scanning microscope (CLSM), Flow cytometry (FCM), Indirect immunofluorescence assay (IFA), Western-blotting (WB), and Quantitative real-time RT-PCR (QRT-PCR) methods were used to explore the effects of inflammation on EGF/EGFR biological activity and signal transduction profiles. We found that the EGF/EGFR nuclear signal almost disappeared in the inflammatory state, and the phosphorylation levels of EGFR, AKT, and STAT3 were all significantly down-regulated. In addition, we also studied the effect of beta-carotene on the biological activity of EGF, and found that when cells were pretreated with beta-carotene, the cellular behavior, biological activity, and nuclear signal of EGF/EGFR under inflammation stimulation were partially restored. In summary, the current study shows that inflammation can disrupt EGF/EGFR-mediated signaling in IEC6 cells, suggesting that inflammation negatively regulates the biological activity of EGF/EGFR. Furthermore, we found that beta-carotene not only attenuated lipopolysaccharide (LPS)-induced inflammation but also partially restored the biological activity of EGF in IEC6 cells, laying a solid foundation for studying the biological functions of EGF and beta-carotene.

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