Identification of the p34 Protein of African Swine Fever Virus as a Novel Viral Antigen with Protection Potential

文献类型： 外文期刊

第一作者： Zhang, Xin

作者： Zhang, Xin;Guan, Xiangyu;Yang, Xiaoke;Li, Shuwen;Zhao, Xiao-Tian;Yuan, Mengqi;Qiu, Hua-Ji;Li, Yongfeng;Zhang, Xin;Wang, Qiuxia;Liu, Xingyou;Wang, Xiao

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关键词： African swine fever virus; p34 protein; antigenicity; humoral and cellular immunity

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

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年卷期： 2024 年 16 卷 1 期

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收录情况： SCI

摘要： African swine fever (ASF) is a highly contagious disease caused by African swine fever virus (ASFV), affecting domestic and wild boars. The polyprotein pp220 of ASFV is responsible for producing the major structural proteins p150, p37, p14, p34, and p5 via proteolytic processing. The p34 protein is the main component of the ASFV core shell. However, the immunologic properties of the p34 protein in vitro and in vivo remain unclear. The results showed that the recombinant p34 protein expressed in prokaryotes and eukaryotes could react with convalescent swine sera to ASFV, suggesting that p34 is an immunogenic protein. Significantly, anti-p34 antibodies were found to inhibit the replication of ASFV in target cells. Furthermore, rabbits immunized with the recombinant C-strain of classical swine fever virus containing p34 produced both anti-p34 humoral and cellular immune responses. In addition, the p34 protein could induce a cell-mediated immune response, and a T-cell epitope on the p34 protein was identified using immunoinformatics and enzyme-linked immunospot (ELIspot) assay. Our study demonstrates that the p34 protein is a novel antigen of ASFV with protective potential.

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