In Vitro Propagation Technology for the Endangered Aquatic Species Nymphoides coronata

文献类型： 外文期刊

第一作者： Lin, Fei

作者： Lin, Fei;Kang, Yong;Li, Yamei;Guo, Yuhua;Yang, Guangsui;Yin, Junmei;Tang, Fenling;Lin, Fei;Eissa, Mamdouh A.;Wang, Wei;Yin, Junmei;Eissa, Mamdouh A.

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关键词： tissue culture; Nymphoides; plant growth regulators; 6-BA; IAA; NAA

期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 11 期

页码：

收录情况： SCI

摘要： Nymphoides coronata is an endangered aquatic plant species with significant medicinal and ecological importance. To preserve N. coronata from going extinct, we need to provide seedlings and efficient multiplication techniques so that it can be extensively studied. This study aimed to identify the most suitable sterilization treatment, growth medium, and substrate for the cultivation and propagation of N. coronata. Ethanol sterilization, fungicide treatment, and sterile water washing were the most important sterilization steps. A combination of 6-benzylaminopurine (6-BA) and indoleacetic acid (IAA) was the most suitable medium for bud induction and shoot proliferation. The use of alpha-naphthaleneacetic acid (NAA) increased the rooting rate and rooting time compared to indole-3-butyric acid (IBA). Increasing the concentration of NAA from 0.5 to 1.0 mg/L increased the rooting rate from 78 to 100% and reduced the rooting time from 7 to 5 days. The survival rate of N. coronata seedlings was 100% in a mixture of red soil and sand (1:1, w/w). As a result, the procedure mentioned above could potentially be used to safely propagate this rare species on a large scale. These findings provide valuable insights into the optimal conditions for the successful cultivation and propagation of N. coronata, which can contribute to the conservation and sustainable use of this important rare plant species.

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