文献类型： 外文期刊
第一作者： Chen, Si
作者： Chen, Si;Chen, Jing;Yao, Yubo;Chen, Si;Yuan, Hongmei;Yang, Xue;Chen, Jing;Liu, Yan;Wu, Linlin;Hu, Yingying;Huang, Wengong;Yao, Yubo;Wu, Jianzhong;Kang, Qinghua;Song, Xixia;Jiang, Weidong;Sheng, Wanmin;Wu, Guangwen;Chen, Le;Wu, Guangwen
作者机构：
关键词： Flax; pasmo; resistance-related genes; biological information analysis; qRT-PCR; levels of expression
期刊名称：JOURNAL OF NATURAL FIBERS （ 影响因子：3.5； 五年影响因子：3.7 ）
ISSN： 1544-0478
年卷期： 2023 年 20 卷 1 期
页码：
收录情况： SCI
摘要： In this study, the flax (Linum usitatissimum L.) genes associated with resistance to the disease pasmo (Septoria linicola (Speg.) Garassini) were mapped using genome resequencing and bulked segregant analysis (BSA) of genomic DNA of the pasmo-resistant parent y62-9, pasmo-susceptible parent y64-5, and F-2 generation segregants. Pasmo resistance genes were identified using Gene Ontology (GO) functional prediction and gene annotation methods. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed the biological information and molecular mechanisms associated with the flax-pathogen interaction. The results of a quantitative real-time PCR analysis revealed that the levels of expression of the three genes Lus10003106, Lus10022077, and Lus10021999 differed between the y62-9 (pasmo-resistant) and y64-5 (pasmo-susceptible) parental flax lines after the inoculation of plants with the pasmo pathogen. Thus, these genes may play key roles in the resistance of flax to pasmo. The results of this study provide a foundation to support future studies of the pathogenesis of flax disease and the discovery and cloning of resistance genes and development of new molecular markers toward the development of pasmo-resistant flax varieties.
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