Simultaneous Determination of Fluoroquinolones, Tetracyclines and Sulfonamides in Chicken Muscle by UPLC-MS-MS

文献类型: 外文期刊

第一作者: Shen, Jianzhong

作者: Shen, Jianzhong;Guo, Liming;Xu, Fei;Xia, Xi;Li, Xiaowei;Ding, Shuangyang;Rao, Qinxiong

作者机构:

关键词: Ultra-performance liquid chromatography-tandem mass spectrometry;Veterinary drugs;Fluoroquinolones;tetracyclines;and sulphonamides in chicken muscle

期刊名称:CHROMATOGRAPHIA ( 影响因子:2.044; 五年影响因子:1.725 )

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收录情况: SCI

摘要: A rapid and sensitive analytical method for the simultaneous determination of four fluoroquinolones, four tetracyclines and six sulfonamides in chicken muscle using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS-MS) has been developed and validated. Samples were extracted with McIlvaine buffer-acetonitrile, defatted with n-hexane, and analyzed by UPLC-MS-MS. Solvent delay technique was applied in the analysis to remove the non-volatile phosphate and carry out farther on-line SPE clean-up. Satisfactory recoveries (55-110%) of all the veterinary drugs were demonstrated in 1, 10 and 20 mu g kg(-1) spiked levels with the overall RSD for intra- and inter-day of 14 analytes less than 18%. The LOD and LOQ were 0.3 and 1.0 mu g kg(-1), respectively. Quantitative results of 103 real samples indicated that the present method was suitable for the quantitative analysis of real samples.

分类号: O65

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