Rapid and quantitative detection of Aspergillus niger Van Tieghem using loop-mediated isothermal amplification assay
文献类型： 外文期刊
第一作者： Dai, Xiaodong
作者： Dai, Xiaodong;Zhang, Xinyou;Cao, Yanyong;Hou, Meiwei;Li, Huimin;Dai, Xiaodong;Cao, Yanyong;Zhang, Xinyou;Yu, Minghui;Li, Jie;Li, Hangyu;Li, Peipei;Wang, Zhenyu
作者机构：
关键词： Aspergillus niger; GOD; Loop-mediated isothermal amplification; Rapid and quantitative detection
期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.2； 五年影响因子：2.5 ）
ISSN： 1125-4653
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Peanut (Arachis hypogaea L.) crown rot and root rot are common diseases caused by Aspergillus niger Van Tieghem. Early and accurate detection of A. niger is key to disease management. In this study, the design of two to five sets of loop-mediated isothermal amplification (LAMP) primers was based on the EglA, GOD, Tub, NRPS, Tan, CbhA, and CbhB genes of A. niger. Of these, primer set GOD-91 was selected for optimization of the three-factor LAMP system: the Bst DNA polymerase concentration, the concentration ratio of the inner and outer primers, and the concentration of Mg2+. In addition, the optimized LAMP reaction system for A. niger detection was validated for specificity, sensitivity, and on-site feasibility. The specificity test showed that A. niger could be specifically detected with the proposed method without cross-amplification of other pathogenic fungi DNA. Moreover, based on the sensitivity test, the lowest detection limit of this reaction system was 5.1 x 10(-7) ng/mu L pAN01 plasmid DNA, after which a standard curve was generated for the quantitative detection of A. niger. The LAMP method was further applied for field sample assessment before and after A. niger infection, successfully detecting A. niger presence in the samples collected in the field. This study yielded a sensitive, specific, and reproducible LAMP system that can be used to assess on-site samples within 45 min. It is an effective approach for the rapid and quantitative detection of A. niger.
分类号：
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Association Studies of Embryogenic Callus Induction Rate in Peanut (Arachis hypogaea L.)
作者：Luo, Dandan;Qu, Pengyu;Fu, Liuyang;Luo, Dandan;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Liu, Hongfei;Xue, Lulu;Li, Xiaona;Liu, Han;Qu, Pengyu;Zhao, Huanhuan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Dai, Xiaodong;Dong, Wenzhao;Zheng, Zheng;Huang, Bingyan;Zhang, Xinyou;Shi, Lei;Sun, Ziqi;Qi, Feiyan;Zhang, Xinyou;Liu, Hongfei;Li, Xiaona;Liu, Han;Zhang, Xinyou
关键词：peanut; CIR; GWAS; candidate gene
-
Drought Stress Induced Different Response Mechanisms in Three Dendrobium Species under Different Photosynthetic Pathways
作者：Xia, Ke;Wu, Qiaofen;Yang, Yanni;Liu, Qiao;Zhao, Zhiguo;He, Jinxiang;Chai, Shengfeng;Qiu, Shuo;Wang, Zaihua;Li, Jie
关键词：carbon metabolism; drought tolerant; phosphoenolpyruvate carboxylase; enrichment analysis; Dendrobium
-
NIa-Pro of sugarcane mosaic virus targets Corn Cysteine Protease 1 (CCP1) to undermine salicylic acid-mediated defense in maize
作者：Yuan, Wen;Chen, Xi;Du, Kaitong;Jiang, Tong;Li, Mengfei;Fan, Zaifeng;Zhou, Tao;Yuan, Wen;Chen, Xi;Du, Kaitong;Jiang, Tong;Li, Mengfei;Fan, Zaifeng;Zhou, Tao;Cao, Yanyong;Li, Xiangdong;Doehlemann, Gunther
关键词：
-
Transcriptome sequencing and expression analysis in peanut reveal the potential mechanism response to Ralstonia solanacearum infection
作者：Wang, Xiao;Zhang, Xinyou;Wang, Xiao;Qi, Feiyan;Sun, Ziqi;Liu, Hongfei;Wu, Yue;Wu, Xiaohui;Xu, Jing;Liu, Hua;Qin, Li;Dong, Wenzhao;Huang, Bingyan;Zheng, Zheng;Zhang, Xinyou;Wang, Zhenyu;Sang, Suling
关键词：Peanut; Bacterial wilt; Full-length transcriptome; RNA-sequencing; Plant-pathogen pathway; Glutathione metabolism; Candidate gene
-
Comparison of soluble dietary fibers from various quinoa microgreens: Structural characteristics and bioactive properties
作者：Wu, Ding -Tao;Li, Jie;Wang, Jin;Lei, Jing;Hu, Yi-Chen;Wu, Xiao-Yong;Zou, Liang;Wu, Ding -Tao;Li, Jie;Wang, Jin;Lei, Jing;Gan, Ren-You;Qin, Peiyou
关键词：Quinoa microgreen; Soluble dietary fiber; Pectic -polysaccharide; Molecular weights; Constituent monosaccharide; Antioxidant effect; Antiglycation effect; Prebiotic effect; Immunoregulatory effect
-
Genome-wide association study and development of molecular markers for yield and quality traits in peanut (Arachis hypogaea L.)
作者：Guo, Minjie;Deng, Li;Gu, Jianzhong;Miao, Jianli;Yin, Junhua;Li, Yang;Lu, Zhenhua;Li, Shaowei;Hu, Junping;Ren, Li;Fang, Yuanjin;Huang, Bingyan;Sun, Ziqi;Qi, Feiyan;Dong, Wenzhao;Zhang, Xinyou
关键词：Peanut; Yield traits; Quality traits; Re-sequencing; Genome-wide association study (GWAS); Kompetitive allele-specific PCR (KASP) marker
-
Genome-Wide Characterization of the Phenylalanine Ammonia-Lyase Gene Family and Their Potential Roles in Response to Aspergillus flavus L. Infection in Cultivated Peanut (Arachis hypogaea L.)
作者：Chai, Pengpei;Cui, Mengjie;Zhao, Qi;Guo, Tengda;Guo, Jingkun;Wu, Chendi;Du, Pei;Han, Suoyi;Chen, Linjie;Liu, Hua;Xu, Jing;Zheng, Zheng;Huang, Bingyan;Dong, Wenzhao;Zhang, Xinyou
关键词：phenylalanine ammonia-lyase; peanut; A. flavus; phylogenetic analysis; cis-element analysis; expression analysis
