A fluorescent lateral flow immunoassay based on CdSe/CdS/ZnS quantum dots for sensitive detection of olaquindox in feedstuff

文献类型： 外文期刊

第一作者： Hu, Mei

作者： Hu, Mei;Cheng, Yongxia;Yu, Xiaoling;Huang, Xianqing;Li, Yu;Hu, Xiaofei;Wang, Guoqiang

作者机构：

关键词： Olaquindox detection; CdSe; CdS; ZnS quantum dots; Lateral flow immunoassay

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 419 卷

页码：

收录情况： SCI

摘要： A portable fluorescence immunosensor based on the CdSe/CdS/ZnS quantum dots (QDs) with multiple-shell structure was fabricated for the precise quantification of olaquindox (OLA). The QDs labeled anti-OLA anti-body used as bioprobe played an important role in the design and preparation of a lateral flow test strip. Due to the strong fluorescent intensity of QDs, the sensitivity is greatly improved. The quantitative results were obtained using a fluorescent strip scan reader within 8 min, and the calculated limit of detection for OLA at 0.12 mu g/kg, which was 2.7 times more sensitive than that of the conventional colloidal gold-based strips method. Acceptable recovery of 85.0%-95.5% was obtained by the spiked samples. This newly established QDs-based strip immu-noassay method is suitable for the on-site detection and rapid initial screening of OLA in swine feedstuff, and is potentially applied for the detection of other veterinary drugs to ensure food safety.

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