Selection of a Single Chain Variable Fragment Antibody against Ivermectin from a Phage Displayed Library

文献类型： 外文期刊

第一作者： Wen, Shuang

作者： Wen, Shuang;Zhang, Xiao;Liu, Yuan;Zhang, Qingqing;Liu, Xianjin;Wen, Shuang;Liang, Jiansheng

作者机构：

关键词： IVM;scFv;detection;phage displayed library

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Single chain variable fragment antibodies (scFvs) have considerable potential in immunological detection of pesticides, veterinary and other residues. In this study, a large human synthetic phage displayed library was used to select scFvs against ivermectin (IVM) by subtractive panning. After four rounds of panning, seven monoclonal phage particles capable of binding with the IVM were obtained. One of the identified novel anti-IVM scFv, hslVM8, was expressed in Escherichia coli HB2151 and purified with Ni metal ion affinity chromatography. Sodium dodecyl sulfate polyacry-lamine gel electrophoresis (SDS-PAGE) indicated that the relative molecular mass of hslVM8 was estimated at 28 kDa. The purified hslVM8 was used to develop a competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) for IVM. The linear range of detection for standards in this ci-ELISA was approximately 0.1-5 μg/mL and 50% inhibition of control (IC_(50)) was 4.11 μg/mL for IVM. These results showed promising applications of hslVM8 for detecting IVM in agricultural and environmental matrices.

分类号： R15

  • 相关文献

[1]Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin. Zhang, Xiao,Liu, Yuan,Zhang, Cunzheng,Wang, Yun,Liu, Xianjin,Zhang, Xiao,Wang, Yun,Xu, Chongxin. 2012

[2]Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries. Wang, Yun,Zhang, Xiao,Liu, Xianjin,Wang, Yun,Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Liu, Xianjin.

[3]Effect of cumulus-oocyte complexes (COCs) culture duration on in vitro maturation and parthenogenetic development of pig oocyte. Zheng, Xin-Min,Wei, Qing-Xin,Wang, Ya-Gang,Liu, Xi-Mei,Li, Li,Xiao, Hong-Wei,Qiao, Xian-Feng,Hua, Zai-Dong,Xu, Hou-Qiang. 2011

[4]Construction of scFv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library. Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Yu, Xiangyang,Liu, Xianjin. 2010

[5]Functional and binding characterization of a single chain Fv antibody to abscisic acid and conjugated abscisic acid. Chen, Xiaojiao,Tan, Guiyu,Liu, Wei,Wang, Baomin,Zhang, Mingcai,Li, Zhaohu,Lu, Yao,Cao, Zhen.

[6]Established a new double antibodies Sandwich enzyme-linked immunosorbent assay for detecting Bacillus thuringiensis (Bt) Cry1Ab toxin based single-chain variable fragments from a naive mouse phage displayed library. Zhang, Xiao,Xu, Chongxin,Zhang, Cunzheng,Liu, Yuan,Xie, Yajing,Liu, Xianjin. 2014

[7]Expression and Functional Properties of an Anti-Triazophos High-Affinity Single-Chain Variable Fragment Antibody with Specific Lambda Light Chain. Liu, Rui,Liang, Xiao,Xiang, Dandan,Guo, Yirong,Zhu, Guonian,Liang, Xiao,Liu, Yihua. 2016

[8]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[9]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[10]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[11]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[12]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[13]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[14]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[15]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[16]Development of cross-priming amplification coupled with vertical flow visualization for rapid detection of infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish, Siniperca chuatsi. Liu, Wenzhi,Zhou, Yong,Fan, Yuding,Jiang, Nan,Zeng, Lingbing,Cain, Kenneth,Cain, Kenneth. 2018

[17]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[18]The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Liu, Zezhong,Zhao, Furong,Gao, Shandian,Shao, Junjun,Chang, Huiyun. 2016

[19]A Miniature Handheld Automatic Detected Device for Register. Zhang Tiefeng,Wang Jun,Sun Zhihui,Zhao Bo,Lu Guotao. 2011

[20]DEVELOPMENT OF A SIMPLE AND EFFECTIVE METHOD FOR SPECIFIC DETECTION OF PEPPER MILD MOTTLE VIRUS. Liu, Y.,Wang, X.,Zhou, G.,Li, X.. 2009

作者其他论文 更多>>

微信小程序