QTL analysis of low-temperature tolerance in maize germination by SLAF-seq and BSA techniqueq,qq

文献类型： 外文期刊

第一作者： Abc, Tao Yu

作者： Abc, Tao Yu;Ma, Xuena;Cao, Shiliang;Li, Wenyue;Yang, Gengbin;Li, Sinan;Abc, Tao Yu;Cao, Shiliang;Yang, Gengbin;Li, Sinan;Abc, Tao Yu;Cao, Shiliang;Li, Sinan

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关键词： Germination; Low temperature tolerance; Maize; QTL analysis; Stress

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.3； 五年影响因子：2.5 ）

ISSN： 0717-3458

年卷期： 2024 年 70 卷

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收录情况： SCI

摘要： Background: Cold damage of maize during germination is a global problem; it occurs frequently in northeast China, and leads to a large-scale reduction in yield. Low temperature tolerance of maize in germination is a complex quantitative trait controlled by multigenes, and no major QTLs or key genes have been identified. Results: An F2 isolation population with S319 and R144 as parents was constructed. The bulked segregant analysis (BSA) and specific-locus amplified fragment-sequencing (SLAF-seq) methods were applied to locate the chromosomal association regions related to low-temperature tolerance of maize during germination. Sequencing obtained 221.72 Gbp clean data, with an average sequencing depth of 25.96X. Four candidate regions associated with low-temperature tolerance trait of maize in germination were obtained, with a total length of 25.71 Mb and 1513 annotated genes, including 456 nonsynonymous mutant genes and 111 frameshift mutant genes.

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