Modulating of d-band center in Cu single-atom anchored Co3O4 nanozyme for sensitive immunochromatographic test strip

文献类型： 外文期刊

第一作者： Zhang, Cui

作者： Zhang, Cui;Dong, Saiyu;Liu, Nairu;Zhang, Xi;Dong, Jie;Zhou, Xueping;Wu, Jianxiang;Zhang, Cui;Wu, Jianxiang;Liu, Zhaoli;Zhou, Xueping;Guo, Yirong

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关键词： Immunochromatographic test strip; Nanozyme; D-band center; Orbital hybridization; Xanthomonas oryzae pv. oryzicola

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）

ISSN： 1385-8947

年卷期： 2024 年 499 卷

页码：

收录情况： SCI

摘要： Immunochromatographic test strip (ITS) has been widely employed for point-of-care diagnoses. However, how to significantly enhance the analytical sensitivity of device-free ITS remains a challenge. Here, we synthesized a Cu single-atom anchored Co3O4 (CuSA/Co3O4) nanozyme that possesses ultrahigh peroxidase-mimicking catalytic activity owing to d-band center upshift mediated by Cu single-atom. Molecular orbital analysis demonstrated that d orbitals in CuSA/Co3O4 have stronger orbital hybridization affinity than Co3O4 to H2O2 ' s pi* orbitals. This reinforces hydroxyl radical (OH) production and provides a stronger catalytic colorimetric signal, which is beneficial for improving the sensitivity of ITS. Moreover, we applied the CuSA/Co3O4 to ITS as a signal reporter for monitoring Xanthomonas oryzae pv. oryzicola (Xoc), the pathogen of rice bacterial leaf streak. The visual limit of detection (LOD) of the developed CuSA/Co3O4-based ITS was as low as 6.4 x 10(2) CFU mL(-1), which is 25-fold more sensitive than the traditional Au nanoparticles (AuNP)-based ITS. Taken together, this work demonstrates the great potential of CuSA/Co3O4 nanocomposite as a signal reporter to improve the sensitivity of ITS for on-site Xoc detection.

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