Virus-induced silencing of genes encoding LEA protein in Tibetan hulless barley (Hordeum vulgare ssp vulgare) and their relationship to drought tolerance

文献类型： 外文期刊

第一作者： Liang, Junjun

作者： Liang, Junjun;Deng, Guangbing;Long, Hai;Pan, Zhifen;Wang, Chunping;Xu, Deling;Yu, Maoqun;Liang, Junjun;Xu, Deling;Cai, Peng;Cai, Peng;Nima, Zha-Xi;Nima, Zha-Xi

作者机构：

关键词： HVA1; Dhn6; Virus-induced gene silencing (VIGS); Barley stripe mosaic virus (BSMV); Water loss rate (WLR)

期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）

ISSN： 1380-3743

年卷期： 2012 年 30 卷 1 期

页码：

收录情况： SCI

摘要： Expression of the late embryogenesis abundant (LEA) gene is usually associated with plant response to dehydration. In this study, a drought-tolerant genotype was screened from 48 accessions of Tibetan hulless barley (Hordeum vulgare ssp. vulgare). By using virus-induced gene silencing, the influence of two LEA genes (HVA1 and Dhn6) on drought tolerance of Tibetan hulless barley was investigated. Results of quantitative real-time PCR indicated that the relative expression levels of HVA1 and Dhn6 in silenced plants were significantly reduced compared with control plants. Both HVA1-silenced and Dhn6-silenced plants showed a consequently lower survival rate than control plants under drought stress. However, only HVA1-silenced plants exhibited a significantly higher water loss rate (WLR). These results suggested that HVA1 and Dhn6 might participate in adaptive responses to water deficit in different ways. Vegetative growth of HVA1-silenced plants was significantly retarded even under optimal growth conditions, and their biomass accumulation was also much lower than that of the controls. These results indicate that HVA1 might play a role in vegetative growth of Tibetan hulless barley.

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