Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity
文献类型： 外文期刊
第一作者： Zhang, C.
作者： Zhang, C.;Song, Y.;Jiang, F.;Jiang, Y.;Zhu, C.;Wen, F.;Li, G.
作者机构：
关键词： Agrobacterium tumefaciens; Nicotiana tabacum; Oryza sativa; Potato virus Y; Rice stripe virus
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
ISSN： 0006-3134
年卷期： 2012 年 56 卷 4 期
页码：
收录情况： SCI
摘要： To induce virus resistance in tobacco and rice we constructed hairpin RNA expression system harbouring inverted repeat fragments of coat protein cDNA of Potato virus Y (PVY) or Rice stripe virus (RSV). These structures were driven by three promoters [cauliflower mosaic virus 35S (CaMV 35S), polyubiqutin gene of maize (Ubi), and Pharbitis nil leucine zipper gene (PNZIP)] which have different tissue-specific activity. PVY resistance ratios were 65.18, 24.33 and 83.54 % in transgenic tobacco plants harboring p35S-PVY, pUbi-PVY and pPNZIP-PVY. RSV resistance was 16.21, 28.61 and 29.33 % in transgenic rice plants harboring p35S-RSV, pUbi-RSV and pPNZIP-RSV. Northern blotting and GUS assay demonstrated that virus resistance levels were related to promoter activity. Therefore, choice of the more effective and tissue-specific promoter to reinforce transcription of hpRNAs will favour the cultivation of highly virusresistant transgenic plants.
分类号： Q94
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period
作者：Qi, J.;Li, J.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Hou, S.
关键词：Serum; biochemical indicator; laying period; GWAS; ducks
-
The gene CmPYL6 strongly contributes to cold tolerance in oriental melon
作者：Liu, W.;Lv, Y.;Zhang, L.;Liu, S.;Zhang, J.;He, M.;Jiang, Y.;Wang, Z.
关键词：ABA; cold stress; oriental melon; PYL
-
Fine mapping and analysis of a candidate gene controlling Phytophthora blight resistance in cucumber
作者：Wang, R.;Zhang, C.;Wu, H.;Guo, J.;Cao, H.;Tan, D.;Wu, T.;Lin, Y. 'e.;Jin, Q.;Wu, T.
关键词：Cucumber blight; fine mapping; Phytophthora melonis; resistance gene
-
Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser)
作者：Yang, Y.;Wang, C.;Liu, Y.;Li, G.;Wang, X.;Wang, H.;He, D.
关键词：Graylag geese; melanin; genetics; dorsal skin; distal feet
-
A High-Density Genetic Map Constructed for Maize (Zea mays L.) Based on Large-Scale SNP Discovery Using Whole-Genome Resequencing and Specific-Locus Amplified Fragments Sequencing (SLAF-Seq)
作者：Chen, Q.;Song, J.;Nie, Z.;Yu, G. R.;Xu, L. Y.;Zhang, J.;Jiang, Y.;Du, W. -P
关键词：maize; high-density genetic map; specific-locus amplified fragments; SNP marker; segregation pattern
-
Metagenomic reconstructions of caecal microbiome in Landes, Roman and Zhedong White geese
作者：Zhu, J.;Song, Y.;Xiao, Y.;Ma, L.;Yang, H.;Wang, X.;Lyu, W.;Zhu, J.;Hu, C.;Zhu, J.;Hu, C.;Zhu, J.;Hu, C.
关键词：Geese; caecal microbiome; metagenome; MAGs; CAZymes; >
-
Partial shade improved the photosynthetic capacity and polysaccharide accumulation of the medicinal plant Bletilla ochracea Schltr.
作者：Yu, X. F.;Ming, X. Y.;Xiong, M.;Yue, L. J.;Yang, L.;Fan, C. Y.;Zhang, C.
关键词：Bletilla ochracea Schltr; chlorophyll fluorescence; photosynthesis; shade
