Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum

文献类型： 外文期刊

第一作者： Jian Gui-Liang

作者： Jian Gui-Liang;Zhang Ying-Tao;Ai Tie-Min

作者机构：

关键词： defensin;expression;Fusarium oxysporum;RT-PCR;Trichosanthes kirilowii;ANTIMICROBIAL PEPTIDES;PLANT DEFENSINS;HOST-DEFENSE;GENE FAMILY;PROTEINS;PEPPER;POTATO

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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收录情况： SCI

摘要： The gene encoding Trichosanthes kirilowii defensin (TDEF1) was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). The newly discovered TDEF1 cDNA contains 231 bp (Genbank accession number DQ526373) and encodes a 76-amino acid protein, which consists of a 29-amino acid signal peptide and a 47-amino acid mature peptide. The partial cDNA, corresponding to the mature peptide coding region of TDEF1, was inserted into bacterial expression vector pET32a(+). Subsequent expression showed that TDEF1 was produced as a 26-kDa fusion protein in the form of inclusion body in Escherichia coli BL21 (DE3). After protein refolding and purification, the fusion TDEF1 displayed an inhibitive activity against the fungal pathogen, Fusarium oxysporum, with EC50 of 247 mu g/ml by means of fungal growth inhibition method.

分类号： Q93

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