Evaluating the effects of Bt protein Vip3Aa on immature Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)

文献类型： 外文期刊

第一作者： Ali, Intazar

作者： Ali, Intazar;Zhang, Shuai;Cui, Jin-Jie;Sajjad, Asif;Khaliq, Ghulam;Bugti, Ghulam Ali;Abbas, Hafiz Tassawar;Basit, Muhammad;Abbas, Naeem

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关键词： Bio-safety evaluation; Dietary exposure test; Propylea japonica; Vip3Aa protein

期刊名称：EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL （ 影响因子：1.995； 五年影响因子：1.115 ）

ISSN： 1110-1768

年卷期： 2021 年 31 卷 1 期

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收录情况： SCI

摘要： Background Genetically modified (GM) crops have become a common tool for managing insect pests of economic importance. However, before their commercialization, bio-safety evaluation on non-target arthropods such as pollinators and biological control agents is a pre-requisite to save services rendered by them in agro-ecosystem. Results In the present experiment, the potential adverse effects of Bt protein (Vip3Aa) were studied on the life table parameters (pupation and emergence rates, 7-day larval weights, larval development time, and adult weight) of a predatory beetle, Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) in the laboratory. P. japonica was exposed to Bt protein incorporated in synthetic diet at the rate of 200 mu g/g of fresh weight of diet. Temporal stability, bioactivity and the uptake of insecticidal protein were confirmed by enzyme-linked immunosorbent assay and a sensitive insect bioassay. The results indicated that pupation and adult emergence rates did not differ significantly from that of negative control (pure synthetic diet). Similarly, the fresh weight of male and female adults emerged in Vip3Aa treatment (2.87 mg and 3.86 mg, respectively) did not vary significantly from that of the negative control treatment (2.66 mg, 3.75 mg). However, the time taken by the larvae to complete their development (12.70 days) was significantly extended than that of the negative control (10.21 days). In contrast, the tested life-table parameters of P. japonica were adversely affected when fed on synthetic diet containing boric acid (positive control) indicating experimental fitness to detect adverse effects. Conclusions On the basis of the results, it is suggested that GM crops expressing Bt protein (Vip3Aa) are safe for non-target insects such as P. japonica and are compatible with other biological programs for the integrated management of insect pests.

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