Flavonoids Composition and Transcriptome Analysis in Herbaceous Peony (Paeonia lactiflora) of Double-Colored Flowers

文献类型： 外文期刊

第一作者： Wang, X.

作者： Wang, X.;Tang, Y. H.;Luan, Y. T.;Zhao, D. Q.;Tao, J.;Zhang, H. C.

作者机构：

关键词： Paeonia lactiflora; double colored flower; flavonoids; transcriptome; qRT-PCR

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.419； 五年影响因子：1.636 ）

ISSN： 1021-4437

年卷期： 2022 年 69 卷 4 期

页码：

收录情况： SCI

摘要： Herbaceous peony (Paeonia lactiflora Pall.) is a traditional flower, famous in China, which owns highly ornamental value due to its various colors, especially double-colored species. However, the researches on color formation of double colored are rare reported. In this study, the P. lactiflora variety 'Hebao Jinlian' with red-yellow double colored petals was used as materials. The anatomical structure and flavonoid components were observed and determined. It was found that colored cells were mainly distributed in the upper and lower epidermis, six kinds of flavonoid components were identified. The total amount of anthocyanins and anthoxanthins decreased gradually with the growth of flowers, and the content in the outer petals was higher than that in the inner petals. Furthermore, by transcriptome sequence, the differential expression genes (DEGs) of outer and inner petals were enriched in flavonoid biosynthesis, phenylpropane biosynthesis and so on at four developmental stages. The accuracy of transcriptome sequence was verified by the expression pattern of DEGs detected by quantitative real-time polymerase chain reaction (qRT-PCR). Combined the analyses of flavonoid assay with transcriptome sequence, the synergistic effect of chalcone synthase (CHS), flavonoid 3-hydroxylase (F3H) and anthocyanindin synthase (ANS) may be the main reason for the formation of red outer petals, while the expression of chalcone isomerase (CHI) and flavonol synthase (FLS) were conducive to the formation of yellow inner petals. The results will lay a foundation for clarifying the color mechanism of double colored petals of P. lactiflora, and also provide a reference for the study of flower color in other ornamental plants.

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