Identification and characterization of an oocyte factor required for sperm decondensation in pig

文献类型： 外文期刊

第一作者： Li, Jingyu

作者： Li, Jingyu;Xie, Bingteng;Wang, Jiaqiang;Zhao, Yanhua;Jiao, Mingxia;Huang, Tianqing;Kong, Qingran;Liu, Zhonghua;Huan, Yanjun

作者机构：

期刊名称：REPRODUCTION （ 影响因子：3.906； 五年影响因子：4.51 ）

ISSN： 1470-1626

年卷期： 2014 年 148 卷 4 期

页码：

收录情况： SCI

摘要： Mammalian oocytes possess factors to support fertilization and embryonic development, but knowledge on these oocyte-specific factors is limited. In the current study, we demonstrated that porcine oocytes with the first polar body collected at 33 h of in vitro maturation sustain IVF with higher sperm decondensation and pronuclear formation rates and support in vitro development with higher cleavage and blastocyst rates, compared with those collected at 42 h (P<0.05). Proteomic analysis performed to clarify the mechanisms underlying the differences in developmental competence between oocytes collected at 33 and 42 h led to the identification of 18 differentially expressed proteins, among which protein disulfide isomerase associated 3 (PDIA3) was selected for further study. Inhibition of maternal PDIA3 via antibody injection disrupted sperm decondensation; conversely, overexpression of PDIA3 in oocytes improved sperm decondensation. In addition, sperm decondensation failure in PDIA3 antibody-injected oocytes was rescued by dithiothreitol, a commonly used disulfide bond reducer. Our results collectively report that maternal PDIA3 plays a crucial role in sperm decondensation by reducing protamine disulfide bonds in porcine oocytes, supporting its utility as a potential tool for oocyte selection in assisted reproduction techniques.

分类号： R339.2

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