Development of colloidal gold-based immunochromatographic assay for rapid detection of Streptococcus suis serotype 2

文献类型： 外文期刊

第一作者： Ju, Ying

作者： Ju, Ying;Hao, Huai-Jie;Xiong, Guo-Hua;Geng, Hong-Ran;Zheng, Yu-Ling;Yang, Yin-Hui;Jiang, Yong-Qiang;Cai, Xue-Hui;Wang, Jing;Ju, Ying;Cao, Yuanyin

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关键词： method specificity;method sensitivity;method development;method stability

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

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收录情况： SCI

摘要： To develop colloidal gold immunochromatographic strips for the direct detection of the Streptococcus suis serotype 2 antigen, colloidal gold was prepared by reduction of a gold salt with sodium citrate and coupled with polyclonal antibody against S. suis serotype 2. The optimal concentrations of the capture antibody and the coating antibody were determined to be 22og/mL and 2.0mg/mL, respectively, and that of the blocking buffer was determined to be 1.5% bovine serum albumin. Different serotypes of S. suis and other related bacteria were used to evaluate the sensitivity, specificity, and stability of the immunochromatographic strips. The detection sensitivity was found to be as high as 10e CFU/mL. There was no cross-reaction of the antibodies with other serotypes of S. suis (except with SS1/2, which shares some common sugar residues or antigenic determinants with serotype 2) and other related bacteria. In conclusion, we developed colloidal gold immunochromatographic strips that had high sensitivity and specificity. This method proved to be feasible, convenient, rapid, and effective for detecting S. suis serotype 2.

分类号： S85

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