Characterization of a Cry1Ac toxin-binding alkaline phosphatase in the midgut from Helicoverpa armigera (Hubner) larvae

文献类型： 外文期刊

第一作者： Wu, Kongming

作者： Wu, Kongming;Liu, Chenxi;Gao, Yulin;Ning, Changming;Gao, Xiwu;Jurat-Fuentes, Juan Luis

作者机构：

关键词： allelic variation

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Midgut membrane-bound alkaline phosphatases (mALP) tethered to the brush border membrane surface by a glycosylphosphatidylinositol (GPI) anchor have been proposed as crucial for Cry1Ac intoxication. In the present work, two full-length cDNAs-encoding alkaline phosphatases in the midgut of Helicoverpa armigera larvae were cloned and named HaALP1 (GenBank accession no. EU729322) and HaALP2 (GenBank accession no. EU729323), respectively. These two clones displayed high identity (above 94%) at the amino acid sequence, indicating that they may represent allelic variants, and were predicted to contain a GPI anchor. Protein sequence alignment revealed that HaALPs were grouped with mALP from the Heliothis virescens midgut. The HaALP1 and HaALP2 (similar to 68 kDa) proteins were heterologously expressed in Sf9 cells using a baculovirus expression system and purified to homogeneity. Ligand blot and dot blot analysis revealed that the Cry1Ac bound to both denatured and native purified HaALPs. Data from lectin blots, competition assays with soybean agglutinin (SBA) lectin and GalNAc binding inhibition assays were indicative of the presence of GalNAc on HaALPs and binding of Cry1Ac toxin to this residue. This observation was further confirmed through N-glycosidase digestion of HaALPs, which resulted in reduced Cry1Ac binding. Our data represent the first report on HaALPs and their putative role as receptors for Cry1Ac toxin in H. armigera.

分类号： Q965

  • 相关文献

[1]Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers. Dongyun Ma,Jun Yan,Zhonghu He,Ling Wu,Xianchun Xia.

[2]Allelic variation, balancing selection and positive selected sites detected from MHC class I alpha gene of olive flounder. Chen, Song-lin,Xu, Tian-jun,Sun, Yue-na. 2010

[3]Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon. Jiang, Yiwei,Pei, Zhongyou,Liu, Huifen,Jiang, Yiwei,Zhao, Xiongwei,Wang, Xicheng,Yu, Xiaoqing,Zhao, Xiongwei,Luo, Na,Garvin, David F.,Garvin, David F.. 2017

[4]Characterization of Tamyb10 allelic variants and development of STS marker for pre-harvest sprouting resistance in Chinese bread wheat. Wang, Y.,Wang, X. L.,Meng, J. Y.,Yang, Y.,Zhang, Y. J.,He, Z. H..

[5]Comparison of amylopectin structure and activities of key starch synthesis enzymes in the grains of rice single-segment substitution lines with different Wx alleles. Teng, Bin,Zhang, Ying,Wu, Jingde,Li, Zefu,Luo, Zhixiang,Yang, Jianbo,Zhang, Chen.

[6]Detection of allelic variation at the Wx locus with single-segment substitution lines in rice (Oryza sativa L.). Teng, Bin,Zeng, Ruizhen,Wang, Yicun,Liu, Ziqiang,Zhang, Zemin,Zhu, Haitao,Ding, Xiaohua,Li, Wentao,Zhang, Guiquan,Teng, Bin,Zeng, Ruizhen,Liu, Ziqiang,Zhang, Zemin,Zhang, Guiquan. 2012

[7]DEVELOPMENT OF STS MARKERS FOR WAXY GENE ALLELIC VARIATION IDENTIFICATION IN FOXTAIL MILLET. Jia, X. P.,Dong, P. H.,Yu, F. Y.,Quan, J. Z.,Bai, H.,Dong, Z. P.. 2015

[8]Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families. Wang YuePing,Qiu LiJuan,Wang YuePing,Chen XiongTing. 2008

[9]Characterization of a New Pm2 Allele Conferring Powdery Mildew Resistance in the Wheat Germplasm Line FG-1. Ma, Pengtao,Xu, Hongxng,Zhang, Hongxia,Han, Guohao,Xu, Yunfeng,Zhang, Xiaotian,An, Diaoguo,Li, Lihui,Fu, Xiaoyi. 2016

[10]Allelic variation of a soluble acid invertase gene (SAI-1) and development of a functional marker in sweet sorghum [Sorghum bicolor (L.) Moench]. Liu, Yang,Nie, Yuan-Dong,Han, Fen-Xia,Zhao, Xiang-Na,Dun, Bao-Qing,Lu, Ming,Li, Gui-Ying,Liu, Yang. 2014

[11]Allelic variations in exon 2 of caprine MHC class II DRB3 gene in Chinese indigenous goats. Li, Meng-Hua,Li, Kui,Kantanen, Juha,Feng, Zheng,Fan, Bin,Zhao, Shu-Hong. 2006

[12]Natural allelic variations of TCS1 play a crucial role in caffeine biosynthesis of tea plant and its related species. Jin, Ji-Qiang,Yao, Ming-Zhe,Ma, Chun-Lei,Ma, Jian-Qiang,Chen, Liang.

[13]Assessing and broadening genetic diversity of a rapeseed germplasm collection. Wu, Jinfeng,Li, Feng,Xu, Kun,Cao, Guizhen,Chen, Biyun,Yan, Cuixin,Wang, Nian,Qiao, Jiangwei,Li, Jun,Li, Hao,Zhang, Tianyao,Song, Weiling,Wu, Xiaoming.

[14]Allelic Variation at the Glu-D3 Locus in Chinese Bread Wheat and Effects on Dough Properties, Pan Bread and Noodle Qualities. Liu, L.,He, Z. H.,Xia, X. C.,He, Z. H.,Ma, W. J.,Liu, J. J.,Pena, R. J..

[15]Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle. Yuan, Zheng Rong,Li, Jiao,Liu, Li,Zhang, Lu Pei,Zhang, Li Min,Chen, Cui,Chen, Xiao Jie,Gao, Xue,Li, Jun Ya,Chen, Jin Bao,Gao, Hui Jiang,Xu, Shang Zhong.

[16]Haplotypic Structure and Allelic Variation of rab17, an ABA-Responsive Gene, in a Mini Core Set of Chinese Diversified Maize Inbred Lines. Yu Yong-tao,Wang Rong-huan,Shi Yun-su,Song Yan-chun,Wang Tian-yu,Li Yu,Yu Yong-tao. 2010

[17]Cloning and allelic variation of two novel catalase genes (SoCAT-1 and SsCAT-1) in Saccharum officinarum L. and Saccharum spontaneum L.. Liu, Yang,Yao, Yanli,Hu, Xiaowen,Xing, Shulian,Xu, Lei,Liu, Yang.

[18]Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat. Zhao, X. L.,Xia, X. C.,He, Z. H.,Lei, Z. S.,Appels, R.,Yang, Y.,Sun, Q. X.,Ma, W..

[19]Allelic variants of phytoene synthase 1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour. He, X. Y.,He, Z. H.,Xia, X. C.,He, Z. H.,Ma, W.,Appels, R.. 2009

[20]Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Liu, L,He, ZH,Yan, J,Zhang, Y,Xia, XC,Pena, RJ. 2005

作者其他论文 更多>>

微信小程序