文献类型： 外文期刊

第一作者： Guo Xian

作者： Guo Xian;Yue Yaojing;Jiao Shuo;Cao Xueliang;Feng Ruilin;Guo Xian;Zhao Xingxu

作者机构：

关键词： twinning induction

期刊名称：JOURNAL OF APPLIED ANIMAL RESEARCH （ 影响因子：1.63； 五年影响因子：1.727 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Xian, G., Yaojing, Y., Shuo, J., Xueliang, C., Ruilin, F. and Xingxu, Z. 2010. Tandem inhibin gene immunization to induce sheep twinning. J. Appl. Anim. Res., 37: 93-96.To study the effect of sheep twinning after tandem inhibin gene immunization, the recombinant plasmid of tandem inhibin were constructed with inhibin a-subunit (1-32) of pig and complement 3d of sheep by RT-PCR, which were used to immunize the sheep. The recombinant plasmid, pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3 were constructed successfully. After BHK-21 cells were transfected with the recombinant plasm id pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3, secreted expression was obtained. After immunization, the twinning rate of sheep was 12.5% and 25.0%, respectively, which was significantly higher (P<0.05) than the control group. The construction of recombinant plasmid of tandem inhibin gene made the theoretical and technical basis for developing the inhibin gene as vaccine for sheep.

分类号： Q959.9