The genetic basis of grain protein content in rice by genome-wide association analysis

文献类型： 外文期刊

第一作者： Chen, Pingli

作者： Chen, Pingli;Lou, Guangming;Wang, Yufu;He, Yuqing;Chen, Pingli;Lou, Guangming;Wang, Yufu;He, Yuqing;Chen, Pingli;Chen, Wengfeng;Fan, Zhilan;Liu, Qing;Sun, Bingrui;Mao, Xingxue;Yu, Hang;Jiang, Liqun;Zhang, Jing;Lv, Shuwei;Xing, Junlian;Pan, Dajian;Li, Chen;Chen, Pingli;Chen, Wengfeng;Fan, Zhilan;Liu, Qing;Sun, Bingrui;Mao, Xingxue;Yu, Hang;Jiang, Liqun;Zhang, Jing;Lv, Shuwei;Xing, Junlian;Pan, Dajian;Li, Chen;He, Yuqing;Chen, Pingli;Chen, Wengfeng;Fan, Zhilan;Liu, Qing;Sun, Bingrui;Mao, Xingxue;Yu, Hang;Jiang, Liqun;Zhang, Jing;Lv, Shuwei;Xing, Junlian;Pan, Dajian;Li, Chen;He, Yuqing;Chen, Pingli;Chen, Wengfeng;Fan, Zhilan;Liu, Qing;Sun, Bingrui;Mao, Xingxue;Yu, Hang;Jiang, Liqun;Zhang, Jing;Lv, Shuwei;Xing, Junlian;Pan, Dajian;Li, Chen;He, Yuqing;Chen, Junxiao

作者机构：

关键词： Oryza sativa; Grain protein content; GWAS; Flo5; Grain quality; CRISPR; Cas9

期刊名称：MOLECULAR BREEDING （ 影响因子：3.297； 五年影响因子：3.011 ）

ISSN： 1380-3743

年卷期： 2023 年 43 卷 1 期

页码：

收录情况： SCI

摘要： The grain protein content (GPC) of rice is an important factor that determines its nutritional, cooking, and eating qualities. To date, although a number of genes affecting GPC have been identified in rice, most of them have been cloned using mutants, and only a few genes have been cloned in the natural population. In this study, 135 significant loci were detected in a genome-wide association study (GWAS), many of which could be repeatedly detected across different years and populations. Four minor quantitative trait loci affecting rice GPC at four significant association loci, qPC2.1, qPC7.1, qPC7.2, and qPC1.1, were further identified and validated in near-isogenic line F-2 populations (NIL-F-2), explaining 9.82, 43.4, 29.2, and 13.6% of the phenotypic variation, respectively. The role of the associated flo5 was evaluated with knockdown mutants, which exhibited both increased grain chalkiness rate and GPC. Three candidate genes in a significant association locus region were analyzed using haplotype and expression profiles. The findings of this study will help elucidate the genetic regulatory network of protein synthesis and accumulation in rice through cloning of GPC genes and provide new insights on dominant alleles for marker-assisted selection in the genetic improvement of rice grain quality.

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