Exosomal microRNA let-7-5p from Taenia pisiformis Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP delta

文献类型： 外文期刊

第一作者： Wang, Liqun

作者： Wang, Liqun;Liu, Tingli;Chen, Guoliang;Li, Yanping;Zhang, Shaohua;Mao, Li;Liang, Panhong;Li, Taoshan;Luo, Xuenong;Harandi, Majid Fasihi;Luo, Xuenong

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关键词： Taenia pisiformis cysticercus; exosome; miRNA let-7-5p; macrophages polarization

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

ISSN：

年卷期： 2021 年 9 卷 7 期

页码：

收录情况： SCI

摘要： Cysticercus pisiformis, the larval stage of Taenia pisiformis, causes serious illness in rabbits that severely impacts the rabbit breeding industry. An inhibitive Th2 immune response can be induced by let-7-enriched exosomes derived from T. pisiformis cysticercus. However, the underlying molecular mechanisms are not completely understood. Here, we report that exosomal miR-let-7-5p released by T. pisiformis cysticercus played a critical role in the activation of M2 macrophages. We found that overexpression of let-7-5p in M1 macrophages decreased M1 phenotype expression while promoting polarization to the M2 phenotype, which is consistent with experimental data in exosome-treated macrophages alone. In contrast, knockdown of let-7-5p in exosome-like vesicles promoted M1 polarization and decreased M2 phenotype expression. Furthermore, down-regulation of transcription factor CCAAT/enhancer-binding protein (C/EBP)-delta resulted in the decrease of M1 phenotype markers and increase of M2 phenotype markers. These results suggested that let-7 enriched in exosome-like vesicles from T. pisiformis metacestodes can induce M2 macrophage polarization via targeting C/EBP delta, which may be involved in macrophage polarization induced by T. pisiformis metacestodes. The finding helps to expand our knowledge of the molecular mechanism of immunosuppression and Th2 immune response induced by metacestodes.

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