Regulation of winter wheat-originated antifreeze glycoprotein on rooster spermatozoa freezability

文献类型： 外文期刊

第一作者： Zong, Yunhe

作者： Zong, Yunhe;Sun, Yanyan;Li, Yunlei;Han, Xintong;Ma, Tianxiao;Zhao, Yi;Yuan, Jingwei;Ma, Hui;Ma, Lin;Chen, Jilan

作者机构：

关键词： chicken; spermatozoa; cryopreservation; antifreeze protein; fertility

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 9 期

页码：

收录情况： SCI

摘要： The freezability of chicken spermatozoa is low, therefore, effective cryoprotectants is desiderated. Antifreeze proteins (AFPs) are widely found in cold-tolerant species and help them to survive in freezing environments. This study was the first to evaluate the effects of different concentrations of plant-originated antifreeze glycoprotein (AFGP) (0, 0.1, 1, and 5 mu g/ mL) on post-thawed sperm motion characteristics, morphology, mitochondrial function, antioxidant activity, and fertilizing potential in chickens. Results showed that the total motility of 0.1 to 1 mu g/mL AFGP groups were significantly higher than those of the 5 mu g/mL AFGP group (P P < 0.05). The post-thawed sperm viability of 0.1 g/mL AFGP group was significantly higher than any of test groups (P P < 0.05). Higher abnormal morphology rate of post-thawed sperm was observed in the control group (0 g/mL AFGP) than in the 0.1, 1, and 5 mu g/mL AFGP groups (P P < 0.05). The concentrations of malondialdehyde (MDA) decreased gradually with the increase of AFGP concentration. ATP was significantly higher in the 0.1 and 1 g/mL AFGP groups than those of control and any of test groups (P P < 0.05). The 0.1 to 1 g/mL AFGP groups had increased mitochondrial membrane potential (MMP) level (P P > 0.05). The 0.1 mu g/mL AFGP group had the highest average fertility (61.36%) compared with control group (57.02%) and any of test groups of chickens at 31 wk of age, and the 1 mu g/mL AFGP group had the highest average fertility (37.72%) compared with control group (21.73%) and any of test groups of chickens at 65 wk of age. In conclusion, the results from this study suggest lower concentration of AFGP (0.1-1 mu g/mL) showed positive effect for sperm function. This study inspires the continuous evaluation and seeking right way of adopting different kinds of AFPs in rooster semen cryopreservation.

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