Exosomal circ_CCDC7/gga-miR-6568-3p/Pax7 axis accelerates the differentiation of chicken embryonic stem cells infected with subgroup J avian leukosis virus

文献类型： 外文期刊

第一作者： Zeng, Xiaona

作者： Zeng, Xiaona;Wang, Ruonan;Chen, Sheng;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Tang, Shengqiu;Dong, Xiaoying;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Wang, Ruonan;Liao, Liqin;Chen, Sheng;Kong, Jie;Chen, Liyi;Li, Yajuan;Shao, Guanming;Zhang, Xinheng;Xie, Qingmei;Zeng, Xiaona;Tang, Shengqiu;Li, Yajuan;Wong, Yung Hou;Wong, Yung Hou

作者机构：

关键词： circRNA; ALV-J; chicken embryonic stem cell

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 8 期

页码：

收录情况： SCI

摘要： Exosome-mediated horizontal and vertical transmission of subgroup J avian leukosis virus ( ALV-J ) in poultry flocks can lead to growth inhibition and severe immunosuppression. However, there are few reports on the early infection of chicken embryonic stem cells ( cESCs ) with ALV-J. In this study, we con firmed that early infection with ALV-J can accelerate the differentiation of cESCs and promote the secretion of exosomes. To investigate the modulation strategy of ALV-J in cESCs, circRNA sequencing was performed for further analysis. A total of 305 differentially expressed circRNAs ( DECs ) were obtained, including 71 upregulated DECs. Circ-CCDC7 was found to be the most upregulated DEC and was assessed by qRT-PCR, with the result consistent with the result of circRNAseq. Based on qRT-PCR, gga-miR-6568-3p was found to be the target of the top 3 DECs, including circ-CCDC7, and the stem cell marker gene Pax7 was identi fied as the target gene of gga-miR-6568-3p. This study demonstrated that exosomal circ-CCDC7/gga-miR-6568-3p/ Pax7 accelerates the differentiation of cESCs after early infection with ALV-J.

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