Expression characteristics of the cyp19a1b aromatase gene and its response to 17β-estradiol treatment in largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

第一作者： Zhang, Dongyun

作者： Zhang, Dongyun;Tian, Taihang;Du, Jinxing;Zhu, Tao;Lei, Caixia;Song, Hongmei;Li, Shengjie;Zhang, Dongyun;Tian, Taihang;Han, Linqiang

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关键词： Largemouth bass; cyp19a1b; Gonad development; 17 beta-Estradiol

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2024 年 50 卷 2 期

页码：

收录情况： SCI

摘要： To investigate the regulatory role of the cyp19a1b aromatase gene in the sexual differentiation of largemouth bass (Micropterus salmoides, LMB), we obtained the full-length cDNA sequence of cyp19a1b using rapid amplification of cDNA ends technique. Tissue expression characteristics and feedback with 17-beta-estradiol (E-2) were determined using quantitative real-time PCR (qRT-PCR), while gonad development was assessed through histological section observations. The cDNA sequence of LMB cyp19a1b was found to be1950 base pairs (bp) in length, including a 5 ' untranslated region of 145 bp, a 3 ' untranslated region of 278 bp, and an open reading frame encoding a protein consisting of 1527 bp that encoded 508 amino acids. The qRT-PCR results indicated that cyp19a1b abundantly expressed in the brain, followed by the gonads, and its expression in the ovaries was significantly higher than that observed in the testes (P < 0.05). After feeding fish with E-2 for 30 days, the expression of cyp19a1b in the pseudo female gonads (XY-F) was significantly higher than that in males (XY-M) (P < 0.05), whereas expression did not differ significantly between XX-F and XY-F fish (P > 0.05). Although the expression of cyp19a1b in XY-F and XX-F fish was not significantly different after 60 days (P>0.05), both exhibited significantly higher levels than that of XY-M fish (P<0.05). Histological sections analysis showed the presence of oogonia in both XY-F and XX-F fish at 30 days, while spermatogonia were observed in XY-M fish. At 60 days, primary oocytes were abundantly observed in both XY-F and XX-F fish, while a few spermatogonia were visible in XY-M fish. At 90 days, the histological sections' results showed that a large number of oocytes were visible in XY-F and XX-F fish. Additionally, the gonads of XY-M fish contained numerous spermatocytes. These results suggest that cyp19a1b plays a pivotal role in the development of ovaries and nervous system development in LMB.

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