Achievements and limitations in genetic transformation and regeneration of oil palm: some molecular aspects

文献类型： 外文期刊

第一作者： Rauf, Saeed

作者： Rauf, Saeed;Wang, Yong;Rauf, Saeed;Ortiz, Rodomiro;Al-Khayri, Jameel M.

作者机构：

关键词： In vitro; Recalcitrant; Screening; Somatic embryo; Transcriptome

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.4； 五年影响因子：2.6 ）

ISSN： 0167-6857

年卷期： 2025 年 162 卷 2 期

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收录情况： SCI

摘要： Oil palm (Elaeis guineensis Jacq.) is crucial for the economy of many tropical countries, and high-quality planting material significantly enhances farmers profitability. Conventional breeding methods for oil palm have been slow and largely unsuccessful. In contrast, transgenic approaches offer an alternative solution to improve plants' responses to biotic and abiotic stresses. Transgene introgression can modify plant phenology, oil yield, and fatty acid composition. This review considers several key aspects of oil palm genetic transformation and regeneration, focusing on its achievements and limitations as follows: (1) selectable and visual markers and their efficacy in transgenic oil palm; (2) transformation efficiency using both biolistic gun and Agrobacterium-mediated transformation methods; (3) transgene incorporation showing success and shortcomings of incorporating genes into oil palm; (4) expression transgene analysis to determine suitability as per breeding goals; (5) somatic embryogenesis to facilitate developing transgenic plants across various genotypes; (6) proteomics and transcriptome analyses to identify genes related to callus induction and somatic embryogenesis in oil palm; (7) epigenetic research on explants showing callus induction and including rapid hypomethylation; and (8) regenerated plant material considering flowering defects in clonal propagated material. In summary, this review article highlights the potential of genetic transformation to enhance oil palm traits, while also emphasizing the challenges that remain in this field. Further research will be necessary to overcome these noted shortcomings and fully realize the benefits of transgenic oil palm.

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