A single-nucleotide mutation of G301A in GaIAA14 confers leaf curling in Gossypium arboreum

文献类型： 外文期刊

第一作者： Miao, Pengfei

作者： Miao, Pengfei;Zhang, Huan;Xu, Yifan;Zhang, Ruowen;Hao, Yunfei;Song, Guoli;Liu, Ji;Miao, Pengfei;Zhang, Huan;Xu, Yifan;Zhang, Ruowen;Hao, Yunfei;Song, Guoli;Liu, Ji

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关键词： Gossypium arboreum; CU; GaIAA14; G301A; curly leaf

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Cotton is a crucial fiber and oil crop, playing a significant role in the textile and food industries. Its yield heavily relies on photosynthesis, a process that primarily occurs in the leaves. Consequently, leaf morphology stands as a vital agronomic trait in cotton breeding. However, research on the molecular mechanisms underlying cotton leaf morphogenesis remains relatively limited. Here we identified a curly leaf mutant (CU) in Gossypium arboreum by ethyl methylsulfonate (EMS) mutagenesis. The genetic analysis revealed that the curly leaf trait in this mutant is a semi-dominant characteristic controlled by a single gene. The map-based cloning of the CU locus showed a single-nucleotide mutation from G to A at the 301st positions in AUX/IAA14 protein (GaIAA14), which resulted in an amino acid substitution from valine (V) to isoleucine (I). After silencing GaIAA14 through virus-induced gene silencing (VIGS) technology in CU mutant, the leaves exhibited a flattened phenotype, indicating that GaIAA14 is a key gene regulating leaf curling in cotton. Comparative transcriptomic RNA-Seq analysis revealed significant changes in the expression levels of most auxin-related genes, suggesting that the mutation disrupts auxin signaling transduction. These findings establish a foundation for further functional studies of this gene and provide research strategies for leaf morphology improvement.

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