Integrating Metabolomics and Transcriptomics to Analyse and Reveal the Regulatory Mechanisms of Mung Bean Polyphenols on Intestinal Cell Damage Under Different Heat Stress Temperatures

文献类型： 外文期刊

第一作者： Feng, Yuchao

作者： Feng, Yuchao;Suo, Decheng;Li, Zetong;Fan, Xia;Feng, Yuchao;Zhang, Shu;Fu, Tianxin;Li, Ying;Wang, Changyuan

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关键词： mung bean polyphenols; heat stress; gut; multi-omics; mechanism

期刊名称：NUTRIENTS （ 影响因子：5.0； 五年影响因子：6.0 ）

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年卷期： 2025 年 17 卷 1 期

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收录情况： SCI

摘要： Background/Objectives: Polyphenols represent a new strategy of dietary intervention for heat stress regulation. Methods: The metabolic and genetic effects of three heat stress-regulated mung bean polyphenols on mouse small intestinal epithelial Mode-k cells were investigated by metabolomics-transcriptomics correlation analysis at different heat stress levels. Results: Lipid metabolism, energy metabolism, and nervous system pathways were the key metabolic regulatory pathways. Under the heat stresses of 39 degrees C, 41 degrees C, and 43 degrees C, the key pathways regulated by mung bean polyphenols on intestinal epithelial Mode-k cells were choline metabolism, pyrimidine metabolism, and the retrograde endorphin signalling pathway in cancer, respectively. FoxO, Rap1, and PI3K-Akt signalling pathways were the key environmental regulatory signalling pathways. Mung bean polyphenols can alleviate heat stress-induced cells at 39 degrees C by inhibiting cell apoptosis and promoting lipid and amino acid accumulation. Mung bean polyphenols can alleviate the threat of cell death caused by heat stress at 41 degrees C by regulating heat shock proteins, inhibiting mitochondrial function and some nerve disease-related genes. The threat of cell death by heat stress at 43 degrees C can be alleviated by regulating nerve-related genes. Conclusions: This study confirmed that mung bean polyphenols can regulate heat stress. The results provide a reference for analysing the mechanism of dietary polyphenol regulating heat stress.

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