A 13-Lipoxygenase, GhLOX2, positively regulates cotton tolerance against Verticillium dahliae through JA-mediated pathway

文献类型： 外文期刊

第一作者： Shaban, Muhammad

作者： Shaban, Muhammad;Noor, Etrat;Malik, Waqas;Ali, Hafiz Muhammad Wasif;Qayyum, Abdul;Khan, Aamir Hamid;Shehzad, Muhammad;Akram, Umar

作者机构：

关键词： Cotton lipoxygenase; Verticillium wilt; RNAi; Gene expression; Lignin

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 796 卷

页码：

收录情况： SCI

摘要： Verticillium wilt is a major limiting factor for sustainable production of cotton but the mechanism of controlling this disease is still poorly understood. Lipoxygenase (LOX)-derived oxylipins have been implicated in defense responses against diverse pathogens; however there is limited information about the functional characterization of LOXs in response to Verticillium dahliae infection. In this study, we report the characterization of a cotton LOX gene, GhLOX2, which phylogenetically clustered into 13-LOX subfamily and is closely related to Arabidopsis LOX2 gene. GhLOX2 was predominantly expressed in leaves and strongly induced following V. dahliae inoculation and treatment of methyl jasmonate (MeJA). RNAi-mediated knock-down of GhLOX2 enhanced cotton susceptibility to V. dahliae and was coupled with suppression of jasmonic acid (JA)-related genes both after inoculation with the cotton defoliating strain V991 or MeJA treatment. Interestingly, lignin contents, transcripts of lignin synthesis genes and H2O2 contents were also decreased in GhLOX2-silenced plants. This study suggests that GhLOX2 is involved in defense responses against infection of V. dahliae in cotton and supports that JA is one of the major defense hormones against this pathogen.

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[1]A 13-Lipoxygenase, GhLOX2, positively regulates cotton tolerance against Verticillium dahliae through JA-mediated pathway. Shaban, Muhammad,Shaban, Muhammad,Noor, Etrat,Malik, Waqas,Ali, Hafiz Muhammad Wasif,Qayyum, Abdul,Khan, Aamir Hamid,Shehzad, Muhammad,Akram, Umar. 2021

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