Determination of tetrodotoxin in puffer fish by ion-paired reagent-assisted dispersive solid-phase extraction (ion-paired-dSPE) in combination with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS)

文献类型： 外文期刊

第一作者： Hou, Chao-Ping

作者： Hou, Chao-Ping;Huang, Ke;Wang, Qiang;Zhang, Ying-Xia;Liu, Qi;Li, Zhi-Guang;Li, Hui-Qing;Wang, Xu-Feng;Hou, Chao-Ping;Huang, Ke;Wang, Qiang;Zhang, Ying-Xia;Liu, Qi;Wang, Xu-Feng;Hou, Chao-Ping;Huang, Ke;Wang, Qiang;Zhang, Ying-Xia;Liu, Qi;Li, Zhi-Guang;Li, Hui-Qing;Wang, Xu-Feng;Huang, Ke;Wang, Xu-Feng;Yang, Jin-Lan;Chen, Hui-Bing

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关键词： Ion-paired reagent assisted dispersive solid phase extraction (Ion-paired-dSPE); Hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS); Tetrodotoxin (TTX); Puffer fish

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2025 年 212 卷

页码：

收录情况： SCI

摘要： Tetrodotoxin (TTX), one of the most potent neurotoxins, poses a significant threat to consumer health. This study presents an advanced analytical method combining ion-paired reagent-assisted dispersive solid-phase extraction (ion-paired-dSPE) with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/ MS), for the quantification of TTX residues in puffer fish. Edible tissue samples were extracted twice using a 1 % formic acid-methanol solution and purified via optimized ion-paired-dSPE procedures. TTX adsorbed onto the magnetic nano-adsorbents was subsequently eluted with an acetonitrile-water-formic acid solution (60:40:1, v/ v/v). Detection was achieved through electrospray ionization in positive mode, with quantification performed in multiple reaction monitoring (MRM) mode. Correlation coefficients (r) in matrix-matched external standard curves ranged from 0.9995 to 0.9997. The limits of detection (LOD) and quantification (LOQ) in puffer fish tissues were 0.1 mu g/kg and 0.3 mu g/kg, respectively. Spiked recoveries at three concentrations in negative tissue samples ranged from 73.4 % to 87.5 %, with precision under 10.6 %. The method was validated in accordance with 2002/657/EC guidelines and successfully applied to TTX detection in cultured puffer fish samples.

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