Identification and Expression Analysis of Lipoxygenase Gene in Bitter Gourd (Momordica charantia)

文献类型： 外文期刊

第一作者： Ge, Haicui

作者： Ge, Haicui;Liu, Shuang;Zheng, Hongzhe;Zhong, Fenglin;Ge, Haicui;Liu, Shuang;Zhong, Fenglin;Chang, Pengyan;Huang, Weiqun;Lin, Shanshan;Zheng, Jingyuan;Li, Honglong;Huang, Zedong;Jia, Qi

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关键词： bitter gourd; McLOX; gene expression

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

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年卷期： 2024 年 15 卷 12 期

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收录情况： SCI

摘要： Background: Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role in the synthesis of fruit aroma volatiles. Methods: LOX gene family members were identified in the whole genome database of bitter gourd and analyzed bioinformatically. An RT-qPCR was used to analyze the expression differences in different tissues. Monoterpenes were determined by gas chromatography-mass spectrometry (GC-MS) technique. Results: A total of 12 LOX gene family members were identified in the genome. The expression of LOX genes varied significantly among the tissues of roots, stems, leaves, flowers, fruits, seeds and tendrils. A total of 29 monoterpenes were detected in the fruits of five different fruit colors of bitter gourd, mainly containing six types of alcohols, aldehydes, terpenes, ketones, esters and alkynes, with the highest relative content of alcohols. Conclusions: The present study provides a reference for further elucidation of the biological functions of the LOX gene in the synthesis pathway of aroma volatiles in bitter gourd.

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