Diversity analysis of nitrite reductase genes (nirS) in black soil under different long-term fertilization conditions
文献类型: 外文期刊
第一作者: Tang, Hui
作者: Tang, Hui;Chi, Fengqin;Wei, Dan;Tang, Hui;Tang, Hui;Yan, Kai;Zhang, Liping;Li, Qing;Lian, Shujuan
作者机构:
关键词: Diversity;Long-term fertilization;NirS genes
期刊名称:ANNALS OF MICROBIOLOGY ( 影响因子:2.112; 五年影响因子:2.151 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A 30-year fertilizer experiment was conducted to investigate the effects of organic manure and mineral NPK fertilizer on black soil. Four experimental treatments were used: CK, organic manure, mineral NPK fertilizer, and organic manure plus mineral fertilizer. The diversity of the nirS gene was analyzed by PCR-restriction fragment length polymorphism. The Shannon diversity index and Evenness factor, Simpson's diversity and Shannon-Weiner indices, principal component analysis and a one-way analysis of similarities test showed that organic manure made the opposite contribution to soil diversity of nirS genes compared to mineral fertilizer treatment, which resulted in increased diversity of the nirS gene. Comparison of the phylogenetic analysis of nirS genes in the mineral fertilizer and organic manure clone libraries showed that induction by mineral fertilizer was stronger than that by organic manure, based on the types of unique nirS genotypes found in the two libraries and the evolutionary distances between them.
分类号: Q93
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