Downregulation of miR-122 by porcine reproductive and respiratory syndrome virus promotes viral replication by targeting SOCS3

文献类型： 外文期刊

第一作者： Zhang, Jing

作者： Zhang, Jing;Li, Fengjuan;Sun, Pu;Wang, Jian;Li, Kun;Zhao, Zhixun;Bai, Xingwen;Cao, Yimei;Bao, Huifang;Li, Dong;Zhang, Jie;Liu, Zaixin;Lu, Zengjun

作者机构：

关键词： PRRSV; MicroRNA; MiR-122; SOCS3; Interferon

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）

ISSN： 0378-1135

年卷期： 2022 年 275 卷

页码：

收录情况： SCI

摘要： MicroRNAs are small non-coding RNA that regulate host anti-viral immune response. In this study, we used high -throughput sequencing to identify miRNAs that were differentially expressed upon PRRSV infection in porcine alveolar macrophages. We observed that the expression level of miR-122 was decreased upon PRRSV infection. Over-expression of miR-122 remarkably suppressed PRRSV replication, while blockage of endogenous miR-122 enhanced PRRSV replication. Moreover, over-expression of miR-122 reduced the protein level of porcine sup-pressor of cytokine signaling 3 (SOCS3), a negative regulator of JAK-STAT signaling, resulting in enhanced production of type I IFN. Further analysis revealed that miR-122 decreased the expression of SOCS3 at the post -transcription level by targeting the 3' UTR region of SOCS3 mRNA. In conclusion, this study demonstrates that the expression of miR-122 was reduced during PRRSV infection. miR-122 impaired PRRSV replication by pro-moting the production of type I interferon. Our study may provide new insights into understanding PRRSV immune evasion mechanisms.

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