Anti-Bacterial and Anti-Inflammatory Properties of Sophoridine and Its Effect on Diarrhea in Mice

文献类型： 外文期刊

第一作者： Wang, Jiaxue

作者： Wang, Jiaxue;Tao, Hui;Fan, Qiuyu;Wang, Zhenlong;Han, Bing;Wang, Xiumin;Wang, Jingquan;Wang, Jiaxue;Tao, Hui;Fan, Qiuyu;Wang, Zhenlong;Han, Bing;Wang, Xiumin;Wang, Jingquan;Wang, Jiaxue

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关键词： sophoridine; anti-bacterial activity; anti-inflammatory activity; acute intestinal inflammation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 5 期

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收录情况： SCI

摘要： To investigate the anti-bacterial and anti-inflammatory properties of sophoridine and elucidate its mechanism of action, we carried out both in vitro and in vivo experiments. Multiple bacterial strains were utilized to determine the effective concentration of sophoridine in antibacterial and bactericidal assays. Subsequently, LPS-stimulated RAW264.7 cells and E. coli-challenged BALB/c mice models were employed to evaluate the production of inflammatory cytokines. Our results showed that sophoridine concentrations exceeding 5.12 mg/mL significantly inhibited cell viability, while 0.32 mg/mL of sophoridine demonstrated the optimal anti-inflammatory activity at 12 h. In E. coli-induced diarrheal mice, doses of 15, 30, and 60 mg/kg BW of sophoridine alleviated fecal occult blood and exhibited anti-inflammatory effects by reducing the level of serum TNF-alpha, IL-1 beta, and IL-6 levels, increasing serum IL-10, and inhibiting leucocyte infiltration in the duodenum. Notably, 15 mg/kg BW of sophoridine effectively decreased the mRNA and protein expression of NF-kappa B p65. These findings suggest that sophoridine has promising potential for the treatment of diarrhea through its anti-inflammatory effects mediated by the inhibition of NF-kappa B activation.

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