Nanoparticle-dsRNA Treatment of Pollen and Root Systems of Diseased Plants Effectively Reduces the Rate of Tobacco Mosaic Virus in Contemporary Seeds

文献类型： 外文期刊

第一作者： Xu, Xiang

作者： Xu, Xiang;Jiao, Yubing;Shen, Lili;Li, Ying;Yang, Jinguang;Mei, Yunpeng;Yang, Wengang;Li, Changquan;Cao, Yi;Chen, Fenglei;Li, Bin

作者机构：

关键词： tobacco mosaic virus (TMV); RNA interference (RNAi); nanoparticle-dsRNA; chitosan quaternary ammonium salt(HACC); seed-borne pathogens

期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：9.5； 五年影响因子：9.6 ）

ISSN： 1944-8244

年卷期： 2023 年 15 卷 24 期

页码：

收录情况： SCI

摘要： Most crop viruses are carried and spread by seeds. Virus-infectedseeds are seed-borne viral disease infections, and thus, reducingthe rate of seed infection is an urgent problem in the seed-productionindustry. The objective of this study was to use nanoparticles (NPs)to directly deliver dsRNA into plants or pollen to initiate RNA interference(RNAi) to reduce viral carryover in seeds. Chitosan quaternary ammoniumsalt (HACC), complexed with dsRNAs, was selected for targeting thegenes for the tobacco mosaic virus (TMV) coat protein (CP) and TMVRNA-dependent RNA polymerase (RdRP) to form HACC-dsRNA NPs. TheseNP-based dsRNAs were delivered to the plants using four differentmethods, including infiltration, spraying, root soaking, and polleninternalization. All four methods were able to reduce the seed-carryingrate of offspring seeds of the TMV-infected plants, with pollen internalizationbeing the most effective in reducing the TMV-carrying rate from 95.1to 61.1% in the control group. By measuring the plant uptake of fluorescence-labeledNPs and dsRNAs, the transportation of the HACC-dsRNA NPs into theplants was observed, and the uptake of dsRNA in combination with smallRNA sequencing was further confirmed, resulting in the silencing ofhomologous RNA molecules during the topical application. The resultsdemonstrated that the incidence of TMV infection was reduced by variousdegrees via RNAi induction without the need to develop transgenicplants. These results demonstrate the advantages of NP-based RNAitechnology in breeding for disease resistance and developing a newstrategy for virus-resistant breeding in plants.

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