Disruption of the rice ALS1 localized in chloroplast causes seedling-lethal albino phenotype

文献类型： 外文期刊

第一作者： Xu, Yibo

作者： Xu, Yibo;Shen, Wei;Zhou, Haiyu;He, Xinhua;Li, Rongbai;Qin, Baoxiang;Wu, Zishuai;Li, Hu

作者机构：

关键词： Rice; Chloroplast development; albino and lethal seedling 1 ( als1 ); GTP-binding protein; Translation elongation

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2024 年 338 卷

页码：

收录情况： SCI

摘要： Chloroplasts are the organelles responsible for photosynthesis and regulate normal plant growth. Although translation elongation factors play important roles in chloroplast development, functional studies of chloroplast translation elongation factors in higher plants remain very sparse. Here, we obtained a rice mutant exhibiting seedling-lethal albino phenotype and named it albino and lethal seedling 1 (als1). Consistently, low content of photosynthetic pigments, malformed chloroplasts and defective photosynthesis were observed in als1 mutant leaves. Map-based cloning experiment showed that als1 mutant had a T base insertion in Os02g0595700, causing a frame shift and premature stop codon. ALS1 encoded a GTP-binding protein EF-Tu, which acts as a translation elongation factor in chloroplast protein translation. ALS1 was found to be expressed throughout plant with highest expression level in young leaves. Moreover, ALS1 was located in chloroplast, whereas the truncated als1 could not normally be located in chloroplast. Additionally, the ALS1 mutation significantly influenced the expression of downstream genes, such as genes relevant to chlorophyll biosynthesis, photosynthesis as well as chloroplast development. These results show that ALS1 acts as a key regulator of chloroplast development and plant growth.

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