Competitive fluorescent immunosensor based on catalytic hairpin self-assembly for multiresidue detection of organophosphate pesticides in agricultural products

文献类型： 外文期刊

第一作者： Wang, Yuanshang

作者： Wang, Yuanshang;Wang, Shanshan;Cui, Xueyan;Zhao, Jing;Lei, Xingmei;Xu, Lingyuan;She, Yongxin;Jin, Fen;Wang, Jing;Jin, Maojun;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Eun, Jong-Bang;Shim, Jae -Han;Hammock, Bruce D.;Hammock, Bruce D.

作者机构：

关键词： Catalyzed hairpin self -assembly; Fluorescent immunosensors; Multiresidue detection; Organophosphate pesticides; Gold nanoparticles

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 413 卷

页码：

收录情况： SCI

摘要： Simple and rapid multiresidue trace detection of organophosphate pesticides (OPs) is extremely important for various reasons, including food safety, environmental monitoring, and national health. Here, a catalytic hairpin self-assembly (CHA)-based competitive fluorescent immunosensor was developed to detect OPs in agricultural products, involving enabled dual signal amplification followed by a CHA reaction. The developed method could detect 0.01-50 ng/mL triazophos, parathion, and chlorpyrifos, with limits of detection (LODs) of 0.012, 0.0057, and 0.0074 ng/mL, respectively. The spiked recoveries of samples measured using this assay ranged from 82.8 % to 110.6 %, with CV values ranging between 5.5 % and 18.5 %. This finding suggests that the CHA-based competitive fluorescent immunosensor is a reliable and accurate method for detecting OPs in agricultural products. The results correlated well with those obtained from the liquid chromatography-tandem mass spec-trometry (LC-MS/MS) method, indicating that the CHA-based biosensor is able to accurately detect OPs and can be used as a reliable alternative to the LC-MS/MS method. Additionally, the CHA-based biosensor is simpler and faster than LC-MS/MS, which makes it a more practical and cost-effective option for the detection of OPs. In summary, the CHA-based competitive fluorescent immunosensor can be considered a promising approach for trace analysis and multiresidue determination of pesticides, which can open up new horizons in the fields of food safety, environmental monitoring, and national health.

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