Minor envelope proteins from GP2a to GP4 contribute to the spread pattern and yield of type 2 PRRSV in MARC-145 cells

文献类型： 外文期刊

第一作者： Bai, Yuan-Zhe

作者： Bai, Yuan-Zhe;Sun, Yue;Liu, Yong-Gang;Zhang, Hong-Liang;An, Tong-Qing;Wang, Qian;Tian, Zhi-Jun;Cai, Xue-Hui;Tang, Yan-Dong;Bai, Yuan-Zhe;Qiao, Xinyuan;Cai, Xue-Hui;Tang, Yan-Dong;Tang, Yan-Dong

作者机构：

关键词： spread pattern; PRRSV; cell-to-cell; cell-free; yield

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.7； 五年影响因子：5.9 ）

ISSN： 2235-2988

年卷期： 2024 年 14 卷

页码：

收录情况： SCI

摘要： In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated and live attenuated vaccines (LAVs), are produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in MARC-145 cells have a low yield. Here, we used two type 2 PRRSV strains (CH-1R and HuN4) to identify the genes responsible for virus yield in MARC-145 cells. Our findings indicate that the two viruses have different spread patterns, which ultimately determine their yield. By replacing the viral envelope genes with a reverse genetics system, we discovered that the minor envelope proteins, from GP2a to GP4, play a crucial role in determining the spread pattern and yield of type 2 PRRSV in MARC-145 cells. The cell-free transmission pattern of type 2 PRRSV appears to be more efficient than the cell-to-cell transmission pattern. Overall, these findings suggest that GP2a to GP4 contributes to the spread pattern and yield of type 2 PRRSV.

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