The rubber tree RALF peptide hormone and its receptor protein kinase FER implicates in rubber production

文献类型： 外文期刊

第一作者： Sui, Jinlei

作者： Sui, Jinlei;Fan, Yujie;Zhu, Jinheng;Zhou, Binhui;Tang, Chaorong;Sui, Jinlei;Xiao, Xiaohu;Yang, Jianghua;Fan, Yujie;Zhu, Jinheng;Zhou, Binhui;Zhu, Sirui;Yu, Feng;Sui, Jinlei

作者机构：

关键词： Hevea brasiliensis; RALF-FER; Interaction; Expression; Latex pH; Rubber biosynthesis

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2023 年 326 卷

页码：

收录情况： SCI

摘要： RAPID ALKALINIZATION FACTORs (RALFs), which are secreted peptides serving as extracellular signals transduced to the inside of the cell, interact with the receptor-like kinase FERONIA (FER) and participates in various biological pathways. Here, we identified 23 RALF and 2 FER genes in Hevea brasiliensis (para rubber tree), and characterized their expression patterns in different tissues, across the process of leaf development, and in response to the rubber yield-stimulating treatments of tapping and ethylene. Four Hevea latex (the cytoplasm of rubber-producing laticifers)-abundant RALF isoforms, HbRALF19, HbRALF3, HbRALF22, and HbRALF16 were listed with descending expression levels. Of the four HbRALFs, expressions of HbRALF3 were markedly regulated in an opposite way by the treatments of tapping (depression) and ethylene (stimulation). All of the four latex -abundant RALFs specifically interacted with the extracellular domain of HbFER1. Transgenic Arabidopsis plants overexpressing these HbRALFs displayed phenotypes similar to those reported for AtRALFs, such as shorter roots, smaller plant architecture, and delayed flowering. The application of HbRALF3 and HbRALF19 recombinant proteins significantly reduced the pH of Hevea latex, an important factor regulating latex meta-bolism. An in vitro rubber biosynthesis assay in a mixture of latex cytosol (C-serum) revealed a positive role of HbFER1 in rubber biosynthesis. Taken together, these data provide evidence for the participation of the HbRALF-FER module in rubber production.

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