Diverse amdoparvoviruses infection of farmed Asian badgers (Meles meles)

文献类型： 外文期刊

第一作者： Wu, Yanhong

作者： Wu, Yanhong;Zhu, Yanzhu;Shao, Xiqun;Zhao, Yongqiang;Zhang, Xiuting;Wei, Tao;Peng, Qianwen;Liu, Zongyue;Wang, Jianke

作者机构：

关键词： Aleutian mink disease virus; Meles meles amdoparvovirus (MMADV); Asian badger; infection

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.5； 五年影响因子：2.2 ）

ISSN： 0304-8608

年卷期： 2024 年 169 卷 7 期

页码：

收录情况： SCI

摘要： Amdoparvoviruses infect various carnivores, including mustelids, canids, skunks, and felids. Aleutian mink disease virus (AMDV) belongs to the prototypical species Amdoparvovirus carnivoran1. Here, we identified a novel amdoparvovirus in farmed Asian badgers (Meles meles), and we named this virus "Meles meles amdoparvovirus" (MMADV). A total of 146 clinical samples were collected from 134 individual badgers, and 30.6% (41/134) of the sampled badgers tested positive for amdoparvovirus by PCR. Viral DNA was detected in feces, blood, spleen, liver, lung, and adipose tissue from these animals. Viral sequences from eight samples were determined, five of which represented nearly full-length genome sequences (4,237-4,265 nt). Six serum samples tested positive by PCR, CIEP, and IAT, four of which had high antibody titers (> 512) against AMDV-G. Twenty-six of the 41 amdoparvovirus-positive badgers showed signs of illness, and necropsy revealed lesions in their organs. Sequence comparisons and phylogenetic analysis of the viral NS1 and VP2 genes of these badger amdoparvoviruses showed that their NS1 proteins shared 62.6%-88.8% sequence identity with known amdoparvoviruses, and they clustered phylogenetically into two related clades. The VP2 proteins shared 76.6%-97.2% identity and clustered into two clades, one of which included raccoon dog and arctic fox amdoparvovirus (RFAV), and the other of which did not include other known amdoparvoviruses. According to the NS1-protein-based criterion for parvovirus species demarcation, the MMADV isolate from farm YS should be classified as a member of a new species of the genus Amdoparvovirus. In summary, we have discovered a novel MMADV and other badger amdoparvoviruses that naturally infect Asian badgers and are possibly pathogenic in badgers.

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