Lowering whole cost for sugarcane-based ethanol production by engineered Zymomonas mobilis

文献类型： 外文期刊

第一作者： Dai, Yonghua

作者： Dai, Yonghua;Wu, Bo;Liu, Panting;Chen, Mao;Song, Chao;Gou, Qiyu;Liu, Renbin;Xu, Yansheng;Hu, Guoquan;He, Mingxiong;Dai, Yonghua;Liu, Panting;Chen, Mao;Song, Chao;Gou, Qiyu;Liu, Renbin;He, Mingxiong

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关键词： bioethanol; CRISPR-Cas; fed-batch fermentation; nitrogen source; sugarcane juice; Zymomonas mobilis

期刊名称：GLOBAL CHANGE BIOLOGY BIOENERGY （ 影响因子：4.745； 五年影响因子：5.672 ）

ISSN： 1757-1693

年卷期： 2021 年 13 卷 12 期

页码：

收录情况： SCI

摘要： Lowering cost will prompt the sustainable development of sugarcane-based ethanol industry. In this work, we developed a low-cost process for ethanol production from sugarcane by a genetically engineered Zymomonas mobilis. Fermentation media were first optimized, resulting in a 15.54% increase in ethanol fermentation efficiency as compared to control media. To further reduce the byproduct levan formation, a levansucrase-encoding gene of Z. mobilis, sacB, was deleted through the type I-F CRISPR-Cas system, which resulted in a further elevation of both ethanol conversion ratio and productivity comparing with the starting strain ZMS912 (87.50% vs. 76.77%, 1.95 g/L/h vs. 1.71 g/L/h). Moreover, we conducted fed-batch fermentation for ethanol production using sugarcane juice in 5 L bioreactors and employing the optimized media and engineered strain. The results showed that maximum ethanol titer of 81.59 g/L and productivity of 5.83 g/L/h were achieved. Finally, preliminary techno-economic assessment demonstrated that our efforts to modify media and strain could reduce the processing cost of ethanol production from sugarcane juice, which provides the feasibility for economic ethanol production in the future.

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