Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17 beta-Estradiol-Degrading Bacterium

文献类型： 外文期刊

第一作者： Hao, Peng

作者： Hao, Peng;Wu, Sicheng;Zhang, Xiqing;Gao, Yunhang;Gou, Changlong;Wang, Yuqiong;Wang, Lixia;Zhu, Yanbin;Basang, Wangdui

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关键词： 17 beta-estradiol; Microbacterium resistens MZT7; biodegradation; metabolic pathway; genome analyses

期刊名称：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH （ 影响因子：4.614； 五年影响因子：4.798 ）

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年卷期： 2022 年 19 卷 17 期

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收录情况： SCI

摘要： Due to the ecotoxicity of 17 beta-estradiol (E2), residual E2 in the environment poses potential risks to human and animal health and ecosystems. Biodegradation is considered one of the most effective strategies to remove E2 from the environment. Here, a novel, efficient E2-degrading bacterial strain Microbacterium resistens MZT7 was isolated from activated sludge and characterized. The genome of strain MZT7 contained 4,011,347 bp nucleotides with 71.26% G + C content and 3785 coding genes. There was 86.7% transformation efficiency of 10 mg/L E2 by strain MZT7 after incubation for 5 d at optimal temperature (30 degrees C) and pH (7.0). This strain was highly tolerant to ranges in pH (5.0-11.0), temperature (20-40 degrees C), and salinity (2-8%). Adding sources of carbon (glucose, maltose, sucrose, or lactose) or nitrogen sources (urea, peptone, or beef extract) promoted the degradation of E2 by strain MZT7. However, when yeast extract was added as a nitrogen source, the degradation efficiency of E2 was inhibited. Metabolites were analyzed by LC-MS and three metabolic pathways of E2 degradation were proposed. Further, the intermediates dehydroepiandrosterone and androsta-1,4-diene-3,17-dione were detected, as well as identification of kshB and fadD3 genes by KEGG, confirming one E2 degradation pathway. This study provided some insights into E2 biodegradation.

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