Membrane Interactions of GET1 and GET2 Facilitate Fiber Cell Initiation through the Guided Entry of the TA Protein Pathway in Cotton

文献类型： 外文期刊

第一作者： Liu, Yang

作者： Liu, Yang;Wei, Zhenzhen;Pei, Yayue;Zhang, Tianen;Miao, Pengfei;Gan, Lei;Liu, Ji;Yang, Zuoren;Peng, Jun;Li, Fuguang;Wang, Zhi;Liu, Yang;Yang, Lu;Zhang, Tianen;Miao, Pengfei;Liu, Ji;Peng, Jun;Li, Fuguang;Wang, Zhi;Liu, Yang;Pei, Yanfei;Zou, Xianyan

作者机构：

关键词： GET pathway; GET1; GET2; phylogenetic; cotton fiber

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 44 期

页码：

收录情况： SCI

摘要： The guided entry of TA proteins (GET) pathway, which is responsible for the post-translational targeting and insertion of the tail-anchored (TA) protein into the endoplasmic reticulum (ER), plays an important role in physiological processes such as protein sorting, vesicle trafficking, cell apoptosis, and enzymatic reactions in which the GET1/2 complex is indispensable. However, a comprehensive study of the GET1 and GET2 genes and the GET pathway in cotton has not yet been carried out. Here, 12 GET1 and 21 GET2 genes were identified in nine representative plant species, and the phylogenetic relationships, gene structures, protein motifs, cis-regulatory elements (CREs), and temporal and spatial expression profiles were analyzed thoroughly. Our study indicated that GhGET1s and GhGET2s might be localized on ER membranes. According to expression profiling and CREs analysis, GhGET2-A02 was identified as a promising candidate for fiber cell development, interacting with two GhGET1s in the membrane, with a binding bias toward GhGET1-A06. Silencing of GhGET1-A06 or GhGET2-A02 reduced fiber initiation and elongation. In summary, our research provides important evidence for understanding the gene families and functions of GET1 and GET2 in cotton and provides clues for molecular breeding of high-quality cotton fiber varieties.

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