Functional characterization of a low-density lipoprotein receptor in the lepidopteran model, Bombyx mori

文献类型： 外文期刊

第一作者： Li, Gui-Yun

作者： Li, Gui-Yun;Liu, Xiao-Jing;Li, Mu-Wang;Li, Gui-Yun;Liu, Xiao-Jing;Fang, Gang-Qi;Yang, Jie;Zhan, Shuai;Li, Mu-Wang;Fang, Gang-Qi;Yang, Jie

作者机构：

关键词： Bombyx mori; cholesterol; CRISPR; Cas9; development; megalin; silkworm

期刊名称：INSECT SCIENCE （ 影响因子：3.605； 五年影响因子：3.323 ）

ISSN： 1672-9609

年卷期：

页码：

收录情况： SCI

摘要： The growth and development of metabolous insects are mainly regulated by ecdysone and juvenile hormone. As a member of the low-density lipoprotein receptor (LDLR) family, megalin (mgl) is involved in the lipoprotein transport of cholesterol which is an essential precursor for the synthesis of ecdysone. Despite extensive studies in mammals, the function of mgl is still largely unknown in insects. In this study, we characterize the function of mgl in the silkworm Bombyx mori, the model species of Lepidoptera. We find that mgl is broadly present in the genomes of lepidopteran species and evolved with divergence between lepidopterans and Drosophila. The expression pattern suggests a ubiquitous role of mgl in the growth and development in the silkworm. We further perform clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9-based mutagenesis of Bmmgl and find that both the development and the silk production of the silkworm are seriously affected by the disruption of Bmmgl. Our results not only explore the function of mgl in Lepidoptera but also add to our understanding of how cholesterol metabolism is involved in the development of insects.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Whole-Genome Resequencing Analysis of the Camelus bactrianus (Bactrian Camel) Genome Identifies Mutations and Genes Affecting Milk Production Traits

    作者：Yao, Huaibing;Zhao, Zhongkai;Yang, Jie;Yao, Huaibing;Zhao, Zhongkai;Yang, Jie;Pan, Zhangyuan;Ma, Wanpeng;Su, Zhanqiang

    关键词：Bactrian camel; milk production traits; genomic analysis; SNP; association analysis

  • Studying the role of Bombyx mori molybdenum cofactor sulfurase in Bombyx mori nucleopolyhedrovirus infection

    作者：Lv, Jun-li;Lai, Wen-qing;Gong, Yu-quan;Zheng, Kai-yi;Zhang, Xiao-ying;Wang, Xue-yang;Li, Mu-wang;Wang, Xue-yang;Li, Mu-wang;Dai, Li-shang;Wang, Xue-yang;Li, Mu-wang

    关键词：BmMoCoS; BmNPV; Bombyx mori; response mechanism; uric acid pathway

  • Identification and Transcriptome Analysis of a Novel Allelic Mutant of NAL1 in Rice

    作者：Wang, Yang;Xu, Wanxin;Liu, Yan;Yang, Jie;Guo, Xin;Zhang, Jiaruo;Pu, Jisong;Zhang, Wenfeng;Wang, Yang;Zhang, Wenfeng;Chen, Nenggang

    关键词：rice; narrow leaf allelic mutant; gene cloning; NAL1; RNA-Seq

  • Bombyx mori voltage-dependent anion-selective channel induces programmed cell death to defend against Bombyx mori nucleopolyhedrovirus infection

    作者：Lv, Jun-li;Lai, Wen-qing;Gong, Yu-quan;Zheng, Kai-yi;Zhang, Xiao-ying;Lu, Zhan-peng;Li, Mu-wang;Wang, Xue-yang;Li, Mu-wang;Wang, Xue-yang;Dai, Li-shang;Wang, Xue-yang

    关键词：Bombyx mori; BmVDAC; BmNPV; programmed cell death; response mechanism

  • A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle

    作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang

    关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis

  • The genomic history and global migration of a windborne pest

    作者：Hu, Qing-Ling;Zhuo, Ji-Chong;Lu, Jia-Bao;Lu, Gang;Huang, Hai-Jian;Li, Jun-Min;Chen, Jian-Ping;Zhang, Chuan-Xi;Hu, Qing-Ling;Ye, Yu-Xuan;Li, Dan-Ting;Lou, Yi-Han;Zhang, Xiao-Ya;Chen, Xuan;Wang, Si-Liang;Wang, Zhe-Chao;Ansari, Naved Ahmad;Zhu, Zeng-Rong;Heong, Kong Luen;Zhang, Chuan-Xi;Fang, Gang-Qi;Zhang, Yi-Xiang;Zhan, Shuai;Fang, Gang-Qi;Zhang, Yi-Xiang;Zhan, Shuai;Mazlan, Norida;Mazlan, Norida;Oo, San San;Thet, Thet;Sharma, Prem Nidhi;Jauharlina, Jauharlina;Sukorini, Ir Henik;Ibisate, Michael T.;Rahman, S. M. Mizanur;Ansari, Naved Ahmad;Chen, Ai-Dong;Zhu, Zeng-Rong

    关键词：

  • Multi-omic characterization of allele-specific regulatory variation in hybrid pigs

    作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming

    关键词：

微信小程序